Abstract—We studied quantitative changes in the components of GABAergic system in Sprague–Dawley rats using an ultrasound model of a depression-like state induced by chronic action of randomly alternating ultrasonic frequencies of 20–45 kHz. We measured expression of the GABRA1, GABRA2, GABRA3, and GABRB2 genes coding the α1, α2, α3, and β2 subunits of GABA_A-receptors, respectively, in the hippocampus, prefrontal cortex, mesencephalon, and amygdala after one, two, and three weeks of stress. The levels of relative expression of GABRA1, GABRA2, and GABRA3 genes decreased to a various extent in different brain structures depending on the duration of ultrasound exposure. Reduced relative expression of GABRA1 and GABRA3 genes was found after one week of chronic exposure to ultrasound, while the decrease in relative expression of GABRA2 was seen only after three weeks. The relative expression of GABRB2 remained unchanged. Thus, for the first time, we studied the dynamics of gene expression of GABA_A-receptor subunits accompanying different stages of the development of a depression-like state in rats; this helped to reveal the relation between the detected changes and the duration of exposure to stress.

中文翻译：

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慢性暴露于大鼠中诱发抑郁样状态的同时，脑中GABA A受体亚基的基因表达减少。

摘要—我们使用随机交变的20–45 kHz超声频率的慢性作用引起的抑郁样状态的超声模型研究了Sprague–Dawley大鼠GABA能系统成分的定量变化。我们测量了分别编码GABA _A-受体的α1，α2，α3和β2亚基的GABRA1，GABRA2，GABRA3和GABRB2基因在海马，前额叶皮层，中脑和杏仁核中分别经过一，两次和之后的表达。三个星期的压力。GABRA1，GABRA2和GABRA3的相对表达水平取决于超声波暴露的持续时间，不同大脑结构中的基因减少程度不同。的减少相对表达GABRA1和GABRA3基因是长期暴露于超声波一周后发现，而在相对表达减少GABRA2仅三周后看到。GABRB2的相对表达保持不变。因此，我们首次研究了与大鼠抑郁样状态发展的不同阶段相关的GABA _A-受体亚基基因表达的动力学。这有助于揭示检测到的变化与压力持续时间之间的关系。