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Distinct Cerebellar Glycosphingolipid Phenotypes in Wistar and Lewis Rats
Neurochemical Journal ( IF 0.5 ) Pub Date : 2020-03-31 , DOI: 10.1134/s1819712420010122
Jasminka Rešić Karara , Martin Kowalski , Anita Markotić , Tatijana Zemunik , Vedrana Čikeš Čulić

Abstract

Three strains of laboratory rats (Lewis, Wistar and Sprague–Dawley) commonly used for the study of movement disorders differ in gait traits. Sialic acid containing glycosphingolipids, called gangliosides, are abundant in the nervous system, where they affect numerous neurochemical events. Cerebellum plays the key role in the integration of body movements. This study investigated glycosphingolipid phenotypes with the hypothesis that they differ in cerebella of the three rat strains. In addition, forebrain and brain stem glycosphingolipid phenotypes were determined. Total glycosphingolipid fractions (neutral and acidic) were analysed by high performance thin-layer chromatography (HPTLC). Complex gangliosides were detected with HPTLC immunostaining by using cholera toxin B subunit after the neuraminidase pretreatment. The most significant differences were found in the cerebellum glycosphingolipid content. Lewis rats showed three fold higher monohexaosylceramides (galactosylceramide + sulfatide)/gangliosides ratio compared to Wistar rats. On the other hand, the cerebellum of Wistar rats contained an increased content of complex gangliosides GD1b, GT1b and GQ1b, fourfold, twofold and tenfold, respectively, compared to Lewis rats. This study shows that Wistar and Lewis rat strains have pronounced differences in glycosphingolipid content and the composition of cerebellum.


中文翻译:

Wistar和Lewis大鼠的不同小脑糖鞘脂表型

摘要

三种用于运动障碍研究的实验大鼠(Lewis,Wistar和Sprague-Dawley)在步态特征上有所不同。含有神经鞘糖脂的唾液酸含有糖鞘脂,在神经系统中含量很高,它们会影响许多神经化学事件。小脑在整合人体运动中起关键作用。这项研究调查了糖鞘脂表型,假设它们在三种大鼠品系的小脑中不同。此外,确定了前脑和脑干糖鞘脂表型。通过高效薄层色谱(HPTLC)分析总糖鞘脂级分(中性和酸性)。神经氨酸酶预处理后,使用霍乱毒素B亚基通过HPTLC免疫染色检测复杂的神经节苷脂。在小脑糖鞘脂含量上发现了最显着的差异。与Wistar大鼠相比,Lewis大鼠的单六糖神经酰胺(半乳糖神经酰胺+硫化物)/神经节苷脂比率高出三倍。另一方面,与Lewis大鼠相比,Wistar大鼠小脑中神经节苷脂GD1b,GT1b和GQ1b的含量更高,分别为四倍,两倍和十倍。这项研究表明,Wistar和Lewis大鼠品系的鞘糖脂含量和小脑组成存在明显差异。与Lewis大鼠相比,Wistar大鼠小脑中神经节苷脂GD1b,GT1b和GQ1b的含量更高,分别为四倍,两倍和十倍。这项研究表明,Wistar和Lewis大鼠品系的鞘糖脂含量和小脑组成存在明显差异。与Lewis大鼠相比,Wistar大鼠小脑中神经节苷脂GD1b,GT1b和GQ1b的含量更高,分别为四倍,两倍和十倍。这项研究表明,Wistar和Lewis大鼠品系的鞘糖脂含量和小脑组成存在明显差异。
更新日期:2020-03-31
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