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Immobilization of Microbial Cells on Polymeric Matrices Modified by Plasma Treatment
Applied Biochemistry and Microbiology ( IF 0.8 ) Pub Date : 2020-03-30 , DOI: 10.1134/s0003683820020076
O. I. Guliy , V. V. Simakov , O. A. Karavaeva , A. V. Smirnov

Abstract

It is shown that microbial cells can be immobilized on polymer matrices modified via plasma treatment. On the example of microbial cells Azospirillum brasilense Sp 7, A. lipoferum Sp59b, and Escherichia coli XL-1, it was found that the immobilization efficiency of biological objects depends on the film processing time in plasma. Treatment of a polystyrene film in a high-frequency discharge plasma allowed a significant increase in the lifetime of microbial cells immobilized on its surface. It was shown that the optimal time for film processing in plasma was 30 s, the time to immobilize microbial cells was ~ 20 min, and bacteria remained viable for up to 6 months after immobilization. The use of matrices modified with plasma treatment as a carrier for immobilized the microbial cells to production biosensors is proposed.


中文翻译:

将微生物细胞固定在等离子体处理改性的聚合物基质上

摘要

结果表明,微生物细胞可以固定在通过等离子体处理改性的聚合物基质上。在微生物细胞中,巴西固氮螺旋菌Sp 7,曲霉Sp59b和大肠杆菌XL-1,发现生物物体的固定效率取决于在血浆中的膜处理时间。在高频放电等离子体中处理聚苯乙烯薄膜可以显着增加固定在其表面上的微生物细胞的寿命。结果表明,在血浆中进行薄膜处理的最佳时间为30 s,固定化微生物细胞的时间为〜20分钟,并且固定化后细菌可存活长达6个月。提出了使用经等离子体处理修饰的基质作为将微生物细胞固定到生产生物传感器的载体的用途。
更新日期:2020-03-30
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