Abstract

In the present study, the mechanisms of Aspergillus niger AM1 and AM2 resistance to white phosphorus were studied. It was shown that the presence of white phosphorus (P₄) at a concentration of 0.25% in the medium had a marginal impact on the ratio of living to dead cells during fungal cultivation, which indicates a high resistance of the strains to P₄. Observations made with electron microscopy showed an increase in the thickness of the fungal cell wall, which is a barrier to the penetration of white phosphorus. MALDI results revealed the biosynthesis of new protein enzymes that could potentially participate in the neutralization of white phosphorus. In addition, white phosphorus caused activation of the metabolism, accompanied by an increase in the number of mitochondria in the cells.

中文翻译：

---

白磷对黑曲霉存活，细胞形态和蛋白质组的影响

摘要

在本研究中，研究了黑曲霉AM1和AM2对白磷的抗性机理。结果表明，在培养基中浓度为0.25％的白磷（P ₄）的存在对真菌培养过程中活细胞与死细胞的比率有边际影响，这表明菌株对P ₄的高抗性。电子显微镜观察表明真菌细胞壁的厚度增加，这是白磷渗透的障碍。MALDI结果揭示了可能潜在参与中和白磷中和的新蛋白质酶的生物合成。另外，白磷引起代谢的活化，伴随着细胞中线粒体数目的增加。