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The efficiency of Cytochrome P450 gene-based markers in accessing genetic variability of drumstick (Moringa oleifera Lam.) accessions.
Molecular Biology Reports ( IF 2.8 ) Pub Date : 2020-03-31 , DOI: 10.1007/s11033-020-05391-w
R S Drisya Ravi 1 , E A Siril 2 , Bindu R Nair 2
Affiliation  

Drumstick (Moringa oleifera Lam.) is an important vegetable as well as forage crop of arid and semi-arid zones of the tropics. The leaves and pods of the plant are rich sources of minerals and vitamins. In the present work, genetic diversity study of 23 genotypes of M. oleifera collected from Kerala, Tamil Nadu and Karnataka states of India was carried out using seven cytochrome P450 (CytP450) markers. By using seven pairs of CytP450 gene-based markers, 88.25% of polymorphism was recorded among the 23 sampled genotypes. The Polymorphic Information Content (PI), Marker Index (MI) and Resolving Power obtained for seven primers were estimated 0.23, 2.96 and 9.83, respectively. The Unweighted Pair Group Method with Arithmetic mean (UPGMA) dendrogram based on this marker data indicate that genotypes from different geographical regions are placed in the same clusters. The dendrogram and Principal Coordinates Analysis (PCoA) plots derived from the binary data matrices were highly concordant. The investigation, in brief, proved that CytP450 based marker system is efficient in the elucidation of genetic diversity in M. oleifera accessions.

中文翻译:

基于细胞色素P450基因标记的效率获得鼓槌(Moringa oleifera Lam。)种的遗传变异性。

鸡腿(Moringa oleifera Lam。)是热带地区干旱和半干旱地区的重要蔬菜和饲料作物。植物的叶子和豆荚富含矿物质和维生素。在目前的工作中,使用七个细胞色素P450(CytP450)标记对从印度喀拉拉邦,泰米尔纳德邦和卡纳塔克邦收集的23种基因型油橄榄毛虫进行了遗传多样性研究。通过使用七对基于CytP450基因的标记,在23个采样基因型中记录了88.25%的多态性。获得的7个引物的多态信息含量(PI),标记指数(MI)和分辨能力分别估计为0.23、2.96和9.83。基于此标记数据的算术均值(UPGMA)树状图非加权对分组方法表明,来自不同地理区域的基因型位于相同的簇中。从二进制数据矩阵得出的树状图和主坐标分析(PCoA)图高度一致。简而言之,该研究证明了基于CytP450的标记系统在阐明油橄榄分枝杆菌种质的遗传多样性方面是有效的。
更新日期:2020-03-31
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