Effectiveness of liposomes elaborated with rapeseed phospholipid (RP) extracted from a residue of oil processing, stigmasterol (ST) and/or hydrogenated phosphatidylcholine (HPC) for the encapsulation lactoferrin (LF) was studied; lipid membrane of liposomes was characterized (bilayer size, chain conformational order, lateral packing, lipid phase, and morphology) and the protection offered to the encapsulated LF during in vitro digestion was determined. Liposomes composed of RP+STLC(low concentration) showed spherical and irregular vesicles without perforations. Lamellar structure was organized in a liquid-ordered phase with a potential orthorhombic packing. Stability and size of the liposomes were more affected by gastric digestion than intestinal digestion; 67-80% of the initially encapsulated LF remained intact after gastric digestion whereas the percentage was reduced to 16-35% after intestinal digestion. Our results shows that liposomes elaborated with RP, properly combined with other lipids, can be a useful oral delivery system of molecules sensitive to digestive enzymes.

中文翻译：

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菜籽磷脂基脂质体中包封的乳铁蛋白的体外消化研究。

研究了从石油加工残渣中提取的菜籽磷脂（RP），豆甾醇（ST）和/或氢化磷脂酰胆碱（HPC）制成的脂质体对乳铁蛋白（LF）的包封效果。对脂质体的脂质膜进行了表征（双层大小，链构象顺序，侧向堆积，脂质相和形态），并确定了在体外消化过程中对包封的LF的保护作用。由RP + STLC（低浓度）组成的脂质体显示球形和不规则囊泡，无穿孔。层状结构以有序正交堆积的液态有序相组织。胃消化比肠道消化对脂质体的稳定性和大小的影响更大。胃消化后，最初封装的LF中有67-80％完好无损，而肠消化后该百分比降低到16-35％。我们的结果表明，用RP精制的脂质体与其他脂质适当地结合，可以成为对消化酶敏感的分子的有用的口服给药系统。