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Ultrasound-assisted extraction optimization and validation of ultra-performance liquid chromatographic method for the quantification of miquelianin in Cuphea glutinosa leaves.
Talanta ( IF 6.1 ) Pub Date : 2020-03-31 , DOI: 10.1016/j.talanta.2020.120988
Marí C Santos 1 , Mariana Koetz 2 , Andreas S L Mendez 3 , Amélia T Henriques 1
Affiliation  

Cuphea glutinosa is a medicinal species abundant in South of Brazil, known because of its flavonoids, which have pharmacological properties as antioxidant, anti-hypertensive, diuretic, and antimicrobial. The present study aimed to optimize the extraction and validate an ultra-performance liquid chromatographic method coupled to a photodiode array detector (UPLC-PDA) method for the quantification of a chemical marker miquelianin in C. glutinosa leaves. The optimum conditions for the extraction of miquelianin from leaves of C. glutinosa were determined using a fractional factorial design (FFD) and a central composite design (CCD). An UPLC-PDA method was validated, following the ICH guidelines and RDC 166/2017 of ANVISA (Brazil). The extraction-optimization methodology was obtained with the following parameters: plant:solvent 1:60 (w/v), percentage solvent 38% ethanol, 60 min time, five extractions and particle size ≤ 180 μm. The validation parameters of the quantification method were satisfactory. The results revealed a method with excellent selectivity, linearity, precision (repeatability and intermediate precision were below 2.18 and 1.40%, respectively) and accuracy (mean recovery 90.6%). The average content of miquelianin was 1.03%. Briefly, the optimization of the extractive method in the leaves of C. glutinosa increased the concentration of miquelianin in the crude extract and the method was validated according to the current legislation.



中文翻译:

超声波辅助提取优化和超高效液相色谱法定量研究古铜叶中米克林宁的含量。

gluthea glutinosa是巴西南部丰富的药用物种,因其类黄酮而著称,其具有抗氧化剂,抗高血压药,利尿药和抗菌药的药理特性。旨在本研究以优化提取和验证耦合到光电二极管阵列检测器(UPLC-PDA)方法用于在化学标记miquelianin的定量的超高效液相色谱法C.黄叶片。对于miquelianin的从叶中提取的最佳条件C.黄使用分数阶乘设计(FFD)和中央复合设计(CCD)确定。遵循ICH指南和ANVISA(巴西)的RDC 166/2017,验证了UPLC-PDA方法。采用以下参数获得了提取优化方法:植物:溶剂1:60(w / v),溶剂百分比38%的乙醇,60分钟的时间,五次提取且粒径≤180μm。定量方法的验证参数令人满意。结果表明该方法具有优异的选择性,线性,精密度(重复性和中等精密度分别低于2.18和1.40%)和准确性(平均回收率90.6%)。槲皮素的平均含量为1.03%。简要地讲,对C.glutinosa叶片中提取方法的优化 增加了粗提取物中的miquelianin浓度,该方法已根据现行法规进行了验证。

更新日期:2020-03-31
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