The review gives a large overview of the strategies used for protein synthesis by chemoselective peptide segment ligation on a solid support. It discusses also important aspects that remain to be explored to further develop the technology such as the role of the solid support on reactant diffusion rates, on ligation kinetics, as well as on the folding and functionality of the proteins attached to the solid support.

中文翻译：

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固相蛋白质化学合成中的策略和开放性问题。

该综述对在固体支持物上通过化学选择性肽段连接进行蛋白质合成的策略进行了全面概述。它还讨论了仍有待探索以进一步开发该技术的重要方面，例如固体支持物对反应物扩散速率、连接动力学以及附着在固体支持物上的蛋白质的折叠和功能的作用。