BACKGROUND Bathyarchaeota, a newly proposed archaeal phylum, is considered as an important driver of the global carbon cycle. However, due to the great diversity of them, there is limited genomic information that accurately encompasses the metabolic potential of the entire archaeal phylum. RESULTS In the current study, nine metagenome-assembled genomes of Bathyarchaeota from four subgroups were constructed from mangrove sediments, and metatranscriptomes were obtained for evaluating their in situ transcriptional activities. Comparative analyses with reference genomes and the transcripts of functional genes posit an expanded role for Bathyarchaeota in phototrophy, autotrophy, and nitrogen and sulfur cycles, respectively. Notably, the presence of genes for rhodopsins, cobalamin biosynthesis, and the oxygen-dependent metabolic pathways in some Bathyarchaeota subgroup 6 genomes suggest a light-sensing and microoxic lifestyle within this subgroup. CONCLUSIONS The results of this study expand our knowledge of metabolic abilities and diverse lifestyles of Bathyarchaeota, highlighting the crucial role of Bathyarchaeota in geochemical cycle. Video abstract.

中文翻译：

---

在Bathyarchaeota中，光敏，卟啉生物合成，Calvin-Benson-Bassham循环和尿素生产的基因组和转录组学证据。

背景技术Bathyarchaeota，一种新近提出的古细菌门，被认为是全球碳循环的重要驱动力。但是，由于它们的多样性，因此准确地涵盖整个古细菌门的代谢潜力的基因组信息有限。结果在本研究中，从红树林沉积物构建了四个亚组的水生古细菌的九个由基因组组装的基因组，并获得了用于转录的转录组，以评估其原位转录活性。与参考基因组和功能基因的转录本进行的比较分析表明，水生细菌分别在光养，自养以及氮和硫循环中起着扩展的作用。值得注意的是，视紫红质，钴胺素生物合成，并且一些Bathyarchaeota亚组6基因组中的氧依赖性代谢途径表明该亚组内的光感应和微氧生活方式。结论这项研究的结果扩展了我们对水生古细菌的代谢能力和多种生活方式的认识，突出了水生古细菌在地球化学循环中的关键作用。录像摘要。