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Enhancement of tendon-bone interface healing and graft maturation with cylindrical titanium-web (TW) in a miniature swine anterior cruciate ligament reconstruction model: histological and collagen-based analysis
BMC Musculoskeletal Disorders ( IF 2.3 ) Pub Date : 2020-03-31 , DOI: 10.1186/s12891-020-03199-0
Keisho Ryu , Mitsuru Saito , Daisaburo Kurosaka , Seiichiro Kitasato , Toshiyuki Omori , Hiroteru Hayashi , Tomohiro Kayama , Keishi Marumo

Tendon-bone interface healing and ligamentization of the graft in anterior cruciate ligament (ACL) reconstruction with autografts are important factors affecting treatment outcome. This study aimed to investigate the effectiveness of a cylindrical titanium-web (TW) in tendon-bone interface healing and graft maturation in ACL reconstruction. Fourteen mature female CLAWN miniature swine underwent bilateral ACL reconstructions with patellar tendon (PT) autografts. In one limb, the TW/tendon complex was placed into the proximal side of the tibial tunnel. Only the graft was transplanted into the tunnel in the control limb. The proximal side of the graft was sutured into the stump of the native ACL and the distal end was stapled to the tibia. The animals were euthanized at 4 and 15 weeks postoperatively, for histological and biochemical analyses. Microscopic images in TW limbs showed that ingrowth of tendon-like tissue and mineralized bone tissue into the TW connected the bone and the tendon directly. In contrast, fibrous tissue intervened between the bone and tendon in the control limbs. The total amount of collagen cross-links (which defines the strength of collagen fibers) and the maturation of collagen cross-links in TW tendons were significantly higher (p < 0.05) than those of control limbs. There was no significant difference in the ratio of dihydroxy-lysinonorleucine to hydroxy-lysinonorleucine (an indicator of tissue specific collagen maturation) between TW tendons and that of the native PT. TW promoted the maturation and formation of collagen cross-links in the grafted tendon while maintaining the cross-links pattern of native tendon collagen, and enabled direct binding of tendon to bone.

中文翻译:

微型猪前交叉韧带重建模型中圆柱状钛网(TW)增强腱-骨界面愈合和移植物成熟:组织学和基于胶原蛋白的分析

自体移植重建前交叉韧带(ACL)的肌腱-骨界面愈合和韧带化是影响治疗结果的重要因素。这项研究旨在调查在ACL重建中,圆柱钛网(TW)在肌腱-骨界面愈合和移植物成熟中的有效性。十四只成熟的CLAWN雌性小型猪接受了pa骨腱(PT)自体移植的双侧ACL重建。在一个肢体中,TW /肌腱复合体被放置在胫骨隧道的近侧。仅移植物被移植到对照肢体的隧道中。将移植物的近端缝合到天然ACL的树桩中,将远端钉在胫骨上。术后4周和15周对动物实施安乐死,进行组织学和生化分析。TW肢体的显微图像显示,肌腱样组织和矿化的骨组织向内生长到TW中,直接连接了骨骼和肌腱。相反,在对照肢体中,纤维组织介于骨骼和肌腱之间。TW肌腱中胶原蛋白交联的总量(定义胶原蛋白纤维的强度)和胶原蛋白交联的成熟度显着高于对照肢体(p <0.05)。TW肌腱和天然PT肌腱之间的二羟基-赖氨酸正亮氨酸与羟基-赖氨酸正亮氨酸的比率(组织特异性胶原成熟的指标)的比例没有显着差异。TW促进了嫁接肌腱中胶原交联的成熟和形成,同时保持了天然肌腱胶原的交联模式,
更新日期:2020-03-31
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