Although immunoglobulin A nephropathy (IgAN) is one of the foremost primary glomerular disease, treatment of IgAN is still in infancy. Non-invasive biomarkers are urgently needed for IgAN diagnosis. We investigate the difference in expression profiles of exosomal long non-coding-RNAs (lncRNAs) in plasma from IgAN patients compared with their healthy first-degree relatives, which may reveal novel non-invasive IgAN biomarkers. We isolated exosomes from the plasma of both IgAN patients and their healthy first-degree relatives. High-throughput RNA sequencing and real-time quantitative polymerase chain reaction (qRT-PCR) was used to validate lncRNA expression profiles. Pathway enrichment analysis was used to predict their nearest protein-coding genes. lncRNA-G21551 was significantly down-regulated in IgAN patients. Interestingly, the nearest protein-coding gene of lncRNA-G21551 was found to be encoding the low affinity receptor of the Fc segment of immunoglobulin G (FCGR3B). Exosomal lncRNA-G21551, with FCGR3B as the nearest protein-coding gene, was down-regulated in IgAN patients, indicating its potential to serve as a non-invasive biomarker for IgAN.

中文翻译：

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在IgA肾病患者中鉴定差异表达的循环外泌体lncRNA。

尽管免疫球蛋白A肾病（IgAN）是最重要的原发性肾小球疾病之一，但IgAN的治疗仍处于婴儿期。IgAN诊断迫切需要非侵入性生物标志物。我们调查了IgAN患者血浆中外泌体长非编码RNA（lncRNAs）表达谱与健康一级亲属相比的差异，这可能揭示了新的非侵入性IgAN生物标志物。我们从IgAN患者及其健康一级亲属的血浆中分离出外泌体。高通量RNA测序和实时定量聚合酶链反应（qRT-PCR）用于验证lncRNA表达谱。途径富集分析用于预测它们最近的蛋白质编码基因。IgAN患者中的lncRNA-G21551明显下调。有趣的是 发现最接近lncRNA-G21551的蛋白质编码基因编码免疫球蛋白G（FCGR3B）Fc片段的低亲和力受体。带有FCGR3B作为最接近的蛋白质编码基因的外泌体lncRNA-G21551在IgAN患者中被下调，表明其潜力可作为IgAN的非侵入性生物标志物。