miR-92a-3p and oxidative stress are reportedly associated with venous thrombosis. However, the role of miR-92a-3p and oxidative stress in catheter-related thrombosis (CRT) remains ambiguous. Herein, we studied the roles of miR-92a-3p, oxidative stress, and p38-mitogen-activated protein kinase/nuclear factor kappa-B (MAPK/NF-κB) pathway in CRT. Forty-five male rats were randomly and equally divided into control, sham operation, and CRT groups. The rats were sacrificed after 10 days. Reactive oxygen species (ROS), superoxide dismutase (SOD), and malondialdehyde (MDA) levels in the serum were determined by enzyme-linked immunosorbent assay (ELISA). The expression levels of miR-92a-3p, heme oxygenase-1 (HO-1), NF-κB p65, and p38 MAPK in the venous tissues were detected with quantitative polymerase chain reaction (qPCR) and Western blot. Thrombosis was observed only in the CRT group. Compared with the levels in the control and sham operation groups, ROS and MDA significantly increased in the CRT group, but SOD significantly decreased. qPCR and Western blot results showed that miR-92a-3p, HO-1, p38 MAPK, and NF-κB p65 expression was significantly upregulated in the venous tissues of the CRT group. Moreover, miR-92a-3p was positively correlated with HO-1, which was positively correlated with p38 MAPK and NF-κB p65. miR-92a-3p was correlated with oxidative stress in CRT. miR-92a-3p and oxidative stress contributed to endothelial dysfunction and simultaneously was associated with CRT.

中文翻译：

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miR-92a-3p，氧化应激和p38MAPK /NF-κB通路在中心静脉导管相关血栓形成大鼠中的作用

据报道，miR-92a-3p和氧化应激与静脉血栓形成有关。但是，miR-92a-3p和氧化应激在导管相关血栓形成（CRT）中的作用仍然不明确。在本文中，我们研究了miR-92a-3p，氧化应激和p38促丝裂原激活的蛋白激酶/核因子κB（MAPK /NF-κB）通路在CRT中的作用。随机将45只雄性大鼠随机分为对照组，假手术组和CRT组。10天后处死大鼠。通过酶联免疫吸附测定（ELISA）测定血清中的活性氧（ROS），超氧化物歧化酶（SOD）和丙二醛（MDA）水平。用定量聚合酶链反应（qPCR）和Western blot检测miR-92a-3p，血红素加氧酶-1（HO-1），NF-κBp65和p38 MAPK在静脉组织中的表达水平。仅在CRT组中观察到血栓形成。与对照组和假手术组相比，CRT组的ROS和MDA显着升高，但SOD显着降低。qPCR和蛋白质印迹结果表明，在CRT组的静脉组织中，miR-92a-3p，HO-1，p38 MAPK和NF-κBp65的表达显着上调。此外，miR-92a-3p与HO-1正相关，而HO-1与p38 MAPK和NF-κBp65正相关。miR-92a-3p与CRT中的氧化应激相关。miR-92a-3p和氧化应激导致内皮功能障碍，并同时与CRT相关。qPCR和蛋白质印迹结果表明，在CRT组的静脉组织中，miR-92a-3p，HO-1，p38 MAPK和NF-κBp65的表达显着上调。此外，miR-92a-3p与HO-1正相关，而HO-1与p38 MAPK和NF-κBp65正相关。miR-92a-3p与CRT中的氧化应激相关。miR-92a-3p和氧化应激导致内皮功能障碍，并同时与CRT相关。qPCR和蛋白质印迹结果表明，在CRT组的静脉组织中，miR-92a-3p，HO-1，p38 MAPK和NF-κBp65的表达显着上调。此外，miR-92a-3p与HO-1正相关，而HO-1与p38 MAPK和NF-κBp65正相关。miR-92a-3p与CRT中的氧化应激相关。miR-92a-3p和氧化应激导致内皮功能障碍，并同时与CRT相关。