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Surveillance and taxonomic analysis of the coronavirus dominant in pigeons in China.
Transboundary and Emerging Diseases ( IF 4.3 ) Pub Date : 2020-03-12 , DOI: 10.1111/tbed.13541
Qingye Zhuang 1 , Shuo Liu 1 , Xiaochun Zhang 2 , Wenming Jiang 1 , Kaicheng Wang 1 , Suchun Wang 1 , Cheng Peng 1 , Guangyu Hou 1 , Jinping Li 1 , Xiaohui Yu 1 , Liping Yuan 1 , Jingjing Wang 1 , Yang Li 1 , Hualei Liu 1 , Jiming Chen 1, 3
Affiliation  

Coronaviruses (CoVs) are found in humans and a wide variety of wild and domestic animals, and of substantial impact on human and animal health. In poultry, the genetic diversity, evolution, distribution and taxonomy of CoVs dominant in birds other than chickens remain enigmatic. In our previous study, we proposed that the CoVs dominant (i.e. mainly circulating) in ducks (DdCoVs) should represent a novel species, which was different from the one represented by the CoVs dominant in chickens (CdCoVs). In this study, we conducted a large‐scale surveillance of CoVs in chickens, ducks, geese, pigeons and other birds (quails, sparrows and partridges) using a conserved RT‐PCR assay. The surveillance demonstrated that CdCoVs, DdCoVs and the CoVs dominant in pigeons (PdCoVs) belong to different lineages, and they are all prevalent in live poultry markets and the backyard flocks in some regions of China. We further sequenced seven Coronaviridae‐wide conserved domains in their replicase polyprotein pp1ab of seven PdCoVs and found that the genetic distances in these domains between PdCoVs and DdCoVs or CdCoVs are large enough to separate PdCoVs into a novel species, which were different from the ones represented by DdCoVs or CdCoVs within the genus Gammacoronavirus, per the species demarcation criterion of International Committee on Taxonomy of Viruses. This report shed novel insight into the genetic diversity, distribution, evolution and taxonomy of avian CoVs.

中文翻译:

我国鸽子冠状病毒的监测及分类学分析.

冠状病毒(CoV)存在于人类以及多种野生和家养动物中,对人类和动物健康具有重大影响。在家禽中,除鸡以外的鸟类中占主导地位的冠状病毒的遗传多样性、进化、分布和分类仍然是个谜。在我们之前的研究中,我们提出鸭子中占优势(即主要传播)的冠状病毒(DdCoV)应该代表一种新物种,与鸡中占优势的冠状病毒(CdCoV)所代表的物种不同。在这项研究中,我们使用保守的 RT-PCR 检测对鸡、鸭、鹅、鸽子和其他鸟类(鹌鹑、麻雀和鹧鸪)中的 CoV 进行了大规模监测。监测结果显示,CdCoV、DdCoV和鸽子优势冠状病毒(PdCoV)属于不同的谱系,在我国部分地区的活禽市场和散养鸡群中均有流行。我们进一步对 7 个PdCoV 的复制酶多蛋白 pp1ab 中的 7 个冠状病毒科保守结构域进行了测序,发现 PdCoV 与 DdCoV 或 CdCoV 之间的这些结构域的遗传距离足够大,足以将 PdCoV 分成一个新物种,该物种与所代表的物种不同根据国际病毒分类委员会的物种划分标准,由丙型冠状病毒属内的 DdCoV 或 CdCoV 引起。这份报告对禽类冠状病毒的遗传多样性、分布、进化和分类学提供了新的见解。
更新日期:2020-03-12
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