• The CLE (CLAVATA3 /ESR ) gene family, encoding a group of small secretory peptides, plays important roles in cell‐to‐cell communication, thereby controlling a broad spectrum of development processes. The CLE family has been systematically characterized in some plants, but not in Brassica napus .
• In the present study, 116 BnCLE genes were identified in the B. napus genome, including seven unannotated, six incorrectly predicted and five multi‐CLE domain‐encoding genes. These BnCLE members were separated into seven distinct groups based on phylogenetic analysis, which might facilitate the functional characterization of the peptides.
• Further characterization of CLE pre‐propeptides revealed 31 unique CLE peptides from 45 BnCLE genes, which may give rise to distinct roles of BnCLE and expansion of the gene family. The biological activity of these unique CLE dodecamer peptides was tested further through in vitro peptide assays. Variations in several important residues were identified as key contributors to the functional differentiation of BnCLE and expansion of the gene family in B. napus . Expression profile analysis helped to characterize possible functional redundancy and sub‐functionalization among the BnCLE members.
• This study presents a comprehensive overview of the CLE gene family in B. napus and provides a foundation for future evolutionary and functional studies.

中文翻译：

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甘蓝型油菜CLV3 / ESR相关（CLE）基因家族的鉴定和综合分析。

• 的CLE（CLAVATA3 / ESR）基因家族编码的一组小的分泌肽，在细胞-至-细胞通讯重要的作用，由此控制显影工艺的广谱。该CLE家族进行了系统的特点在一些植物，但不是在甘蓝型油菜。
• 在本研究中，在甘蓝型油菜基因组中鉴定出116个BnCLE基因，包括7个未注释的，6个错误预测的和5个多CLE域编码的基因。根据系统发育分析，将这些BnCLE成员分为七个不同的组，这可能有助于肽的功能表征。
• CLE前肽的进一步表征揭示了来自45个BnCLE基因的31种独特的CLE肽，这可能引起BnCLE的独特作用和基因家族的扩展。通过体外肽测定进一步测试了这些独特的CLE十二聚体肽的生物学活性。在几个重要的残基的变化被确定为主要贡献者的功能分化BnCLE和基因家族的扩大油菜。表达谱分析有助于确定BnCLE成员之间可能的功能冗余和亚功能化。
• 这项研究提供了对甘蓝型油菜CLE基因家族的全面概述，并为将来的进化和功能研究提供了基础。