The deactivation effects and mechanism of plasma‐activated water (PAW) against Staphylococcus aureus biofilm and the inhibitory effect on the biofilm regrowth capacity for the surviving S. aureus post PAW treatment were investigated in vitro. Systematic measurements on bacterial cultivability, metabolic capacity, membrane integrity and intracellular reactive oxygen species (ROS) concentration under two different experimental categories were carried out after PAW treatment. Considerable deactivation effects were discovered on biofilm S. aureus on both prolonging the PAW inducement or treatment time. The rising concentration of intracellular ROS and the PAW‐contained RS might exert synergistic effects in deactivating the biofilm S. aureus. Moreover, the biomass, bacterial cultivability and metabolic capacity of the regrown S. aureus biofilm all significantly declined along with the increasing PAW inducement or treatment time. Therefore, except for the short‐term rapid inactivation effects on the biofilm, the PAW treatment could also exert long‐term inhibitory effects on the regeneration of S. aureus biofilm.

中文翻译：

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空气等离子体活化水对金黄色葡萄球菌生物膜的体外抗菌作用及其机理

体外研究了血浆活化水（PAW）对金黄色葡萄球菌生物膜的失活作用和机理以及对PAW处理后存活的金黄色葡萄球菌对生物膜再生能力的抑制作用。PAW处理后，在两个不同的实验类别下，对细菌的可培养性，代谢能力，膜完整性和细胞内活性氧种类（ROS）浓度进行了系统测量。发现生物膜金黄色葡萄球菌 在延长PAW诱导或治疗时间上均具有相当大的失活作用。细胞内ROS和PAW所含RS浓度的升高可能会在金黄色葡萄球菌生物膜失活中发挥协同作用。。此外，再生的金黄色葡萄球菌生物膜的生物量，细菌可培养性和代谢能力都随着PAW诱导时间或治疗时间的增加而显着下降。因此，除了对生物膜的短期快速灭活作用外，PAW处理还可能对金黄色葡萄球菌生物膜的再生产生长期抑制作用。