The insurgence of newly arising, rapidly developing health threats, such as drug‐resistant bacteria and cancers, is one of the most urgent public‐health issues of modern times. This menace calls for the development of sensitive and reliable diagnostic tools to monitor the response of single cells to chemical or pharmaceutical stimuli. Recently, it has been demonstrated that all living organisms oscillate at a nanometric scale and that these oscillations stop as soon as the organisms die. These nanometric scale oscillations can be detected by depositing living cells onto a micro‐fabricated cantilever and by monitoring its displacements with an atomic force microscope‐based electronics. Such devices, named nanomotion sensors, have been employed to determine the resistance profiles of life‐threatening bacteria within minutes, to evaluate, among others, the effect of chemicals on yeast, neurons, and cancer cells. The data obtained so far demonstrate the advantages of nanomotion sensing devices in rapidly characterizing microorganism susceptibility to pharmaceutical agents. Here, we review the key aspects of this technique, presenting its major applications. and detailing its working protocols.

中文翻译：

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生物体纳米运动检测及其特征透视图

新出现的、快速发展的健康威胁，如耐药细菌和癌症，是现代最紧迫的公共卫生问题之一。这种威胁要求开发灵敏可靠的诊断工具来监测单细胞对化学或药物刺激的反应。最近，已经证明所有活的生物体都在纳米尺度上振荡，并且一旦生物体死亡，这些振荡就会停止。这些纳米尺度的振荡可以通过将活细胞沉积在微制造的悬臂上并通过基于原子力显微镜的电子设备监测其位移来检测。这种名为纳米运动传感器的设备已被用于在几分钟内确定危及生命的细菌的耐药性，以评估、其中包括化学品对酵母、神经元和癌细胞的影响。迄今为止获得的数据证明了纳米运动传感装置在快速表征微生物对药剂的敏感性方面的优势。在这里，我们回顾了这项技术的关键方面，介绍了它的主要应用。并详细说明其工作协议。