In the current work, we design a multifunctional hybrid nanocomposite for treating MCF-7 cell lines, which act as a model for breast cancer cells, to overcome the serious side-effects of chemotherapy treatment pathways. ZnO was designed within the nanoscale range using simple wet chemical pathways and via adding TWEEN as a pore directing agent. ZnO@leucovorin was exploited by confining the ZnO nanoparticles within the cavities and across the surface of the leucovorin drug through the Stöber process from mechanical coating. Various characterization techniques were used to confirm the structure and morphology of the obtained composite material. Furthermore, the inhibition (%) activity of different scavenging radicals, the DPPH, GP_x, SOD and ABTS assays commonly performed to characterize the antioxidant activity, was measured. The obtained results show a high antioxidant activity associated with the hybrid material compared to the pure leucovorin itself. Additionally, ZnO@leucovorin displays a high potent cytotoxicity against MCF-7, established using annexin V-flow cytometry. Subsequently, a gene expression study provided information about the growth factor reduction of the HER-2 gene, which in turn has an effect on the apoptotic death of the cancer cells. In addition, RO measurement exploits the rate of oxygen release inside the cell, and showed a suitable and healthy range after 3 days of treatment compared to that in other studies.

中文翻译：

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ZnO @ leucovorin纳米复合材料对MCF-7细胞模型的高细胞毒活性

在当前的工作中，我们设计了一种多功能的杂合纳米复合材料来治疗MCF-7细胞系，该细胞系可作为乳腺癌细胞的模型，以克服化学疗法治疗途径的严重副作用。ZnO使用简单的湿化学途径并通过添加TWEEN作为孔定向剂在纳米范围内设计。ZnO @ leucovorin的开发是通过机械涂覆的Stöber工艺将ZnO纳米颗粒限制在腔内以及整个leucovorin药物的表面。使用各种表征技术来确认所得复合材料的结构和形态。此外，不同清除自由基DPPH，GP _x的抑制（％）活性测量了通常用于表征抗氧化活性的SOD和ABTS分析。所获得的结果表明，与纯亚叶酸蛋白本身相比，杂化材料具有很高的抗氧化活性。此外，ZnO @ leucovorin对膜联蛋白V流式细胞仪建立的针对MCF-7的细胞具有高毒性。随后，一项基因表达研究提供了有关HER-2基因生长因子降低的信息，这反过来又对癌细胞的凋亡死亡产生了影响。此外，RO测量利用了细胞内氧气的释放速率，与其他研究相比，治疗3天后显示出合适的健康范围。