In the present study, sulfamethazine-imprinted polymers have been prepared by bulk polymerization method with a living radical photopolymerization using sulfamethazine (SM₂) as the template molecule, methacrylic acid (MAA) as the functional monomer, ethylene glycol dimethacrylate (EGDMA) as the cross-linker, and azobisisobutyronitrile (AIBN) as the initiator in the porogenic solvent of acetonitrile. After estimating the recognition properties, the imprinting factor of the polymer reached up to 9.3779. Then, the polymer particles were incorporated into a solid-phase extraction (SPE) column for the selective separation and purification of the sulfonamides (SAs), including sulfadiazine (SD), sulfamerazine (SM₁), and SM₂, from edible swine tissues. By using the detection method of high-performance liquid chromatography with the optimum SPE conditions, the limits of detection and limits of quantitation were calculated as 0.7–2.3 μg kg⁻¹ and 3.4–4.6 μg kg⁻¹ in the complex samples, respectively. The recovery of SAs ranged from 85.5 to 88.3%. Compared with the commonly used Waters Oasis HLB® column, the proposed selective SM₂-MI-SPE column has opened a promising new approach for trace enrichment of the residues. It will facilitate sulfonamide residue analysis in animal tissues.

在本研究中，已通过本体聚合方法，以磺胺_二甲基嘧啶（SM ₂）为模板分子，甲基丙烯酸（MAA）为功能单体，乙二醇二甲基丙烯酸酯（EGDMA）为活性自由基光聚合反应，制备了磺胺甲二嗪印迹聚合物。交联剂，并在乙腈的成孔剂中使用偶氮二异丁腈（AIBN）作为引发剂。估计识别性能后，聚合物的印迹因子达到9.3779。然后，将聚合物颗粒掺入固相萃取（SPE）柱中，以选择性分离和纯化磺胺类药物（SAs），包括磺胺嘧啶（SD），磺胺嘧啶（SM ₁）和SM ₂，来自可食用的猪组织。通过使用具有最佳SPE条件的高效液相色谱检测方法，复杂样品中的检出限和定量限分别计算为0.7–2.3μgkg ^-1和3.4–4.6μgkg ^-1。SA的回收率从85.5％到88.3％。与常用的Waters OasisHLB®色谱柱相比，拟议的选择性SM ₂ -MI-SPE色谱柱为残留物的痕量富集开辟了一种有希望的新方法。这将有助于动物组织中磺酰胺残留的分析。