Soybean is an economically important plant, and its production is affected in soils with high salinity levels. It is important to understand the adaptive mechanisms through which plants overcome this kind of stress and to identify potential genes for improving abiotic stress tolerance. RNA-Seq data of two Glycine max cultivars, a drought-sensitive (C08) and a tolerant (Conquista), subjected to different periods of salt stress were analyzed. The transcript expression profile was obtained using a transcriptogram approach, comparing both cultivars and different times of treatment. After 4 h of salt stress, Conquista cultivar had 1400 differentially expressed genes, 647 induced and 753 repressed. Comparative expression revealed that 719 genes share the same pattern of induction or repression between both cultivars. Among them, 393 genes were up- and 326 down-regulated. Salt stress also modified the expression of 54 isoforms of miRNAs in Conquista, by the maturation of 39 different pre-miRNAs. The predicted targets for 12 of those mature miRNAs also have matches with 15 differentially expressed genes from our analyses. We found genes involved in important pathways related to stress adaptation. Genes from both ABA and BR signaling pathways were modulated, with possible crosstalk between them, and with a likely post-transcriptional regulation by miRNAs. Genes related to ethylene biosynthesis, DNA repair, and plastid translation process were those that could be regulated by miRNA.

中文翻译：

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盐胁迫下两个大豆品种的转录分析。

大豆是一种重要的经济植物，在盐度高的土壤中大豆的生产受到影响。重要的是要了解植物克服这种胁迫的适应机制，并确定潜在的基因以改善非生物胁迫耐受性。分析了两个甘氨酸最大品种（干旱敏感型（C08）和耐旱型（Conquista））在不同盐胁迫时期的RNA-Seq数据。使用转录图方法获得了转录本表达谱，比较了品种和不同处理时间。盐胁迫4小时后，Conquista品种具有1400个差异表达基因，647个诱导基因和753个阻遏基因。比较表达显示，两个品种之间有719个基因共享相同的诱导或抑制模式。其中，393个基因被上调而326个基因被下调。盐胁迫还通过39种不同的pre-miRNA的成熟修饰了Conquista中54种miRNA亚型的表达。这些成熟的miRNA的12个预测目标也与我们分析中的15个差异表达基因相匹配。我们发现基因参与与压力适应相关的重要途径。来自ABA和BR信号通路的基因被调节，它们之间可能发生串扰，并且可能由miRNA进行转录后调控。与乙烯生物合成，DNA修复和质体翻译过程有关的基因是可以由miRNA调控的基因。这些成熟的miRNA的12个预测目标也与我们分析中的15个差异表达基因相匹配。我们发现基因参与与压力适应相关的重要途径。来自ABA和BR信号通路的基因被调节，它们之间可能发生串扰，并且可能由miRNA进行转录后调控。与乙烯生物合成，DNA修复和质体翻译过程有关的基因是可以由miRNA调控的基因。这些成熟的miRNA的12个预测目标也与我们分析中的15个差异表达基因相匹配。我们发现基因参与与压力适应相关的重要途径。来自ABA和BR信号通路的基因被调节，它们之间可能发生串扰，并且可能由miRNA进行转录后调控。与乙烯生物合成，DNA修复和质体翻译过程有关的基因是可以由miRNA调控的基因。