Whey, a by-product of cheese making, is a collection of several milk proteins and has functional and nutritional values. Whey protein concentrate (WPC) exhibits various functional effects by glycation. Studies to find sugar-binding sites in a protein having a functional effect are reported. However, it is rarely clear whether it confirms that glycated single protein exhibits the same effect of protein cluster. This study confirmed which protein sites are responsible for the effect of glycated WPC (G-WPC). β-Lactoglobulin (LG) was the major protein of G-WPC and glycated with lactose. The glycated LG increased the nitric oxide and cytokine secretion similar to G-WPC and peptide sequences of active compound was confirmed using the high molecular weight band of G-WPC. The K151 and K157 residues of LG were modified by glycation with sugar in common with G-WPC. These residues of glycated LG potentially contribute to the immune-modulation effect of G-WPC.

乳清是干酪制造的副产品，是几种牛奶蛋白的集合，具有功能和营养价值。浓缩乳清蛋白（WPC）通过糖基化表现出各种功能作用。报道了在蛋白质中发现具有功能作用的糖结合位点的研究。但是，很少确定是否可以确认糖化的单个蛋白质是否具有相同的蛋白质簇效应。这项研究证实了哪些蛋白质位点负责糖化WPC（G-WPC）的作用。β-乳球蛋白（LG）是G-WPC的主要蛋白质，并被乳糖糖化。糖基化的LG增加了类似于G-WPC的一氧化氮和细胞因子的分泌，使用G-WPC的高分子量谱带证实了活性化合物的肽序列。LG的K151和K157残基通过与G-WPC相同的糖基糖基化修饰。糖基化LG的这些残基可能有助于G-WPC的免疫调节作用。