The complete genomic sequence of a putative novel member of the family Secoviridae was determined by high-throughput sequencing of a pineapple accession obtained from the National Plant Germplasm Repository in Hilo, Hawaii. The predicted genome of the putative virus was composed of two RNA molecules of 6,128 and 4,161 nucleotides in length, excluding the poly-A tails. Each genome segment contained one large open reading frame (ORF) that shares homology and phylogenetic identity with members of the family Secoviridae. The presence of this new virus in pineapple was confirmed using RT-PCR and Sanger sequencing from six samples collected in Oahu, Hawaii. The name "pineapple secovirus A" (PSVA) is proposed for this putative new sadwavirus.

中文翻译：

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鉴定和在菠萝中发现的新型狂犬病病毒的完整基因组序列（Ananas comosus）。

通过从夏威夷希洛的国家植物种质资源库获得的菠萝登录号的高通量测序，确定了拟交粘病毒科新成员的完整基因组序列。推定病毒的预测基因组由两个长度分别为6,128和4,161个核苷酸的RNA分子组成，不包括poly-A尾巴。每个基因组片段都包含一个大的开放阅读框（ORF），该开放阅读框与Secoviridae家族的成员具有同源性和系统发育同一性。使用RT-PCR和Sanger测序从夏威夷瓦胡岛（Oahu）收集的六个样品中证实了这种新病毒在菠萝中的存在。对于这种推定的新的萨德瓦病毒，建议使用名称“菠萝脂蛋白A”（PSVA）。