According to the growth pattern, gastric cancer (GC) could be classified into expanding-type GC and infiltrative-type GC (Ming’s classification). The growth pattern of GC is often related to the malignant degree, invasion, metastasis, and other pathological characteristics of tumors. MicroRNAs (miRNAs) play important roles in modulating gene expression during the GC development. In this study, miR-29s were significantly correlated with the gastric carcinogenesis and Ming’s classification. Biological function of miR-29s is most closely related to the pathway of extracellular matrix (ECM)-receptor interaction. ECM structural assembly, cell movement, and cell adhesion are the main functional categories of target genes in this pathway. Among these targets, the COL4A1 gene ranked at the top in the association analysis of combined miR-29s biological function and GC subtype, and miR-29s inhibited its translation by binding to the 3′ UTR region. Infiltrative-type GC cells secrete a higher level of COL4A1 protein than do expanding-type GC cells. The expression of COL4A1 in GC is correlated with clinicopathological features. Downregulation of COL4A1 expression significantly inhibited the migration and invasion of GC cells. High COL4A1 expression was correlated with poor prognosis in survival analysis. The miR-29s regulatory network may affect the development of growth patterns and pathological progress of GC by regulating the function of COL4A1.

根据胃癌的生长方式，可以将胃癌分为扩张型胃癌和浸润型胃癌（Ming's分类）。GC的生长方式通常与肿瘤的恶性程度，侵袭，转移及其他病理特征有关。MicroRNA（miRNA）在GC开发过程中在调节基因表达中起重要作用。在这项研究中，miR-29s与胃癌的发生和Ming的分类显着相关。miR-29s的生物学功能与细胞外基质（ECM）-受体相互作用的途径最密切相关。ECM结构组装，细胞运动和细胞粘附是该途径中靶基因的主要功能类别。在这些目标中，在组合miR-29s生物学功能和GC亚型的关联分析中，COL4A1基因位居首位，而miR-29s通过与3'UTR区结合来抑制其翻译。浸润型GC细胞比扩张型GC细胞分泌更高水平的COL4A1蛋白。GC中COL4A1的表达与临床病理特征相关。下调COL4A1表达可显着抑制GC细胞的迁移和侵袭。高COL4A1表达与生存分析的不良预后相关。miR-29s调控网络可能通过调控COL4A1的功能来影响GC的生长方式和病理进展。浸润型GC细胞比扩张型GC细胞分泌更高水平的COL4A1蛋白。GC中COL4A1的表达与临床病理特征相关。下调COL4A1表达可显着抑制GC细胞的迁移和侵袭。高COL4A1表达与生存分析的不良预后相关。miR-29s调控网络可能通过调节COL4A1的功能来影响生长模式的发展和GC的病理进展。浸润型GC细胞比扩张型GC细胞分泌更高水平的COL4A1蛋白。GC中COL4A1的表达与临床病理特征相关。下调COL4A1表达可显着抑制GC细胞的迁移和侵袭。高COL4A1表达与生存分析的不良预后相关。miR-29s调控网络可能通过调控COL4A1的功能来影响生长模式的发展和GC的病理进展。