Retinoblastoma is a childhood tumor of the retina that is caused mostly by biallelic inactivation of the tumor suppressor gene RB1. To generate a research resource, we abrogated expression of RB1 in H9 hESCs by CRISPR/Cas9 induced deletion of the RB1 promoter, either on one or on both alleles. This enables studies on the role of RB1 loss during differentiation, for example in differentiation towards neural retina. The generation of three isogenic lines per deletion state enables validation of phenotypic results in independent clonal lines.

中文翻译：

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六个等基因克隆H9 hESC系中RB1启动子的双等位基因和单等位基因缺失。

视网膜母细胞瘤是视网膜的儿童期肿瘤，主要是由抑癌基因RB1的双等位基因失活引起的。为了产生研究资源，我们通过CRISPR / Cas9诱导RB1启动子在一个或两个等位基因上的缺失，消除了H9 hESCs中RB1的表达。这使得能够研究在分化过程中，例如在向神经视网膜的分化过程中RB1丢失的作用。每个缺失状态产生三个等基因系使得能够在独立的克隆系中验证表型结果。