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Volatile profiling and UHPLC-QqQ-MS/MS polyphenol analysis of Passiflora leschenaultii DC. fruits and its anti-radical and anti-diabetic properties.
Food Research International ( IF 8.1 ) Pub Date : 2020-03-30 , DOI: 10.1016/j.foodres.2020.109202
Saravanan Shanmugam 1 , Rafael Donizete Dutra Sandes 2 , Murugan Rajan 2 , Maria Terezinha Santos Leite Neta 2 , Bruno Dos Santos Lima 1 , Maria Joseli Melo de Jesus 1 , Marina Denadai 2 , Narendra Narain 2 , Parimelazhagan Thangaraj 3 , Mairim Russo Serafini 1 , Lucindo José Quintans-Júnior 1 , Adriano Antunes de Souza Araújo 1
Affiliation  

Twenty-four phenolic compounds including daidzein, epicatechin and artepillin C were identified in Passiflora leschenaultii DC. fruit by UHPLC-QqQ-MS/MS analysis. The aroma profile has been studied using the HS–SPME/GC–MS which revealed 67 volatile compounds including 13 terpenes, 18 alcoholics, 15 esters, ketones and phenolic acids. Further, the proximate composition, anti-radical and anti-diabetic activities of fruit pulp were also determined. The fresh fruit pulp of P. leschenaultii registered higher total phenolic (691.90 mg GAE/g extract) and tannin (313.81 mg GAE/g extract) contents and it also exhibited very high DPPH (IC50 of 6.69 µg/ml) and ABTS+• (9760.44 µM trolox equivalent/g extract) scavenging activities. The fresh fruit pulp showed a strong inhibition towards the α-Amylase and α-Glucosidase (IC50 of 32.20 and 19.81 µg/mL, respectively) enzymes. Thus, the work stipulates that phenolic compounds rich P. leschenaultii fruit can serve as a potential nutraceutical, antioxidative and anti-diabetic agent in food and pharmaceutical formulations.



中文翻译:

西番莲DC的挥发性成分分析和UHPLC-QqQ-MS / MS多酚分析。水果及其抗自由基和抗糖尿病特性。

二十四酚类化合物包括黄豆苷原,表儿茶素和artepillin C下在鉴定西番莲leschenaultii DC。水果通过UHPLC-QqQ-MS / MS分析。使用HS-SPME / GC-MS对香气特征进行了研究,结果发现67种挥发性化合物,包括13种萜烯,18种酒类,15种酯,酮和酚酸。此外,还确定了果肉的近成分,抗自由基和抗糖尿病活性。P. leschenaultii的新鲜果肉中总酚含量(691.90 mg GAE / g提取物)和单宁(313.81 mg GAE / g提取物)含量较高,并且还显示出很高的DPPH (IC 50为6.69 µg / ml)和ABTS +•(9760.44 µM trolox当量/克提取物)清除活性。新鲜的果肉显示朝向强烈抑制α淀粉酶和α葡萄糖苷酶(IC 50 32.20 19.81和微克/毫升,分别地)的酶。因此,这项工作规定,富含酚类化合物的P. leschenaultii水果可以在食品和药物制剂中用作潜在的营养保健品,抗氧化剂和抗糖尿病药。

更新日期:2020-03-30
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