Abstract Anti-solvent precipitation of zein was employed in conjunction with spray drying to reduce the usage of ethanol and to encapsulate the aqueous extract of green jelly leaf (Cyclea barbata Miers) antioxidants. Different concentrations of zein (0.35, 0.7, 1.05 and 1.4% w/v) and Hydroxypropylmethyl cellulose (HPMC) (5, 10, 15, 20% w/w) in several different formulations were investigated. The spray dried powders were characterised in terms of their encapsulation efficiency, morphology, average particle size and the antioxidant release. Result shows that encapsulation of green jelly leaf antioxidants using zein were possible with encapsulation efficiency between ~50 and 70%, where a higher concentration of zein in feed solution had favoured better encapsulation efficiency. The spray dried powder produced were relatively small (~6–10 μm) with spherical structure. The addition of HPMC also aided in delaying the agglomeration of zein nano-precipitates in the feed solution through its interaction with zein via hydrogen bonding. These results suggested that zein protein could have a potential application to encapsulate water soluble antioxidant for application in high water content food products.

中文翻译：

---

玉米蛋白作为一种水不溶性赋形剂，用于亲水性生物活性物质的喷雾干燥包封

摘要 玉米醇溶蛋白的反溶剂沉淀与喷雾干燥相结合，以减少乙醇的使用并封装绿色果冻叶 (Cyclea barbata Miers) 抗氧化剂的水提取物。研究了几种不同配方中不同浓度的玉米蛋白（0.35、0.7、1.05 和 1.4% w/v）和羟丙基甲基纤维素 (HPMC)（5、10、15、20% w/w）。喷雾干燥粉末的特征在于它们的包封效率、形态、平均粒径和抗氧化剂释放。结果表明，使用玉米醇溶蛋白包封绿色果冻叶抗氧化剂是可能的，包封率在~50% 到 70% 之间，其中饲料溶液中较高浓度的玉米醇溶蛋白有利于更好的包封效率。产生的喷雾干燥粉末相对较小（~6-10 μm），具有球形结构。HPMC 的添加还有助于延迟玉米蛋白纳米沉淀物在进料溶液中通过氢键与玉米蛋白相互作用的聚集。这些结果表明玉米醇溶蛋白可能具有用于封装水溶性抗氧化剂以用于高含水量食品的潜在应用。