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Improving the power generation performances of Gram-positive electricigens by regulating the peptidoglycan layer with lysozyme.
Environmental Research ( IF 8.3 ) Pub Date : 2020-03-30 , DOI: 10.1016/j.envres.2020.109463
Wenrui Shen 1 , Xiaoran Zhao 2 , Xiaoliang Wang 3 , Siqi Yang 3 , Xindi Jia 3 , Xiaodi Yu 3 , Jing Yang 4 , Qinzheng Yang 1 , Huazhang Zhao 5
Affiliation  

The power generation performance of a microbial fuel cell (MFC) greatly depends on the relative amount of electricigens in the anodic microbial community. Running the MFC multiple times can practically enrich the electricigens, and thus improve its power generation efficiency. However, Gram-positive electricigens cannot be enriched well because of their thick non-conductive peptidoglycan layer. Herein, we report a new Gram-positive electricigen enrichment method by regulating the peptidoglycan layer of the bacteria using lysozyme. Lysozyme can partially hydrolyze the peptidoglycans layer of Gram-positive Firmicutes to improve the permeability of cell wall, and thus enhance its electricity generation activity. The stimulation of Gram-positive electricigen endows MFCs a high power generation community structure, which results in the power density 42% higher than that of the control sample. Our work has provided a new and simple method for optimizing the anode community structure by regulating weak electricigens in the community with lysozyme.

中文翻译:

通过用溶菌酶调节肽聚糖层来提高革兰氏阳性电原的发电性能。

微生物燃料电池(MFC)的发电性能在很大程度上取决于阳极微生物群落中电气化试剂的相对含量。多次运行MFC实际上可以丰富电气设备,从而提高其发电效率。然而,由于革兰氏阳性电致密剂的非导电肽聚糖层较厚,因此不能很好地富集。在这里,我们报告了一种新的革兰氏阳性电动富集方法,该方法通过使用溶菌酶调节细菌的肽聚糖层来实现。溶菌酶可以部分水解革兰氏阳性菌的肽聚糖层,从而提高细胞壁的通透性,从而增强其发电活性。革兰氏阳性电的激发赋予了MFCs高发电社区结构,这导致功率密度比对照样品高42%。我们的工作提供了一种新的简单方法,可以通过溶菌酶调节菌体中的弱电物质来优化阳极菌体结构。
更新日期:2020-03-31
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