Salmonella Typhimurium easily forms biofilms on poultry eggshell, which poses a serious threat to the safety of poultry egg. Therefore, this study aims to evaluate the inhibitory mechanism of cold nitrogen plasma (CNP) on Salmonella Typhimurium biofilm and apply it to poultry eggs to reduce the risk of contamination. The results of crystal violet staining showed that CNP can effectively remove biofilm. Electron paramagnetic resonance (EPR) spectra and reactive oxygen species (ROS) concentration analysis showed that CNP reacts with water to generate hydroxyl radicals and stimulate cells to cause an increase in intracellular oxidative stress. After treating biofilm with CNP (600 W, 2min), the metabolic activity of Salmonella Typhimurium decreased by 82.2%, and the extracellular protein, extracellular polysaccharide and extracellular DNA respectively decreased from 68.5 to 12.3 μg/mL, from 32.1 to 8.4 μg/mL, and 26.5 to 8.6 μg/mL qRT-PCR analysis showed that CNP treatment down-regulated the transcription of genes related to extracellular protein, extracellular polysaccharide and extracellular DNA. In addition, CNP could remove Salmonella Typhimurium biofilm on poultry eggshells without significantly affecting sensory quality.

鼠伤寒沙门氏菌很容易在禽蛋壳上形成生物膜，这严重威胁了禽蛋的安全。因此，本研究旨在评估冷氮血浆（CNP）对鼠伤寒沙门氏菌生物膜的抑制机制，并将其应用于禽蛋以降低污染风险。结晶紫染色的结果表明，CNP可以有效去除生物膜。电子顺磁共振（EPR）光谱和活性氧（ROS）浓度分析表明，CNP与水反应生成羟基自由基，刺激细胞引起细胞内氧化应激的增加。用CNP（600 W，2min）处理生物膜后，沙门氏菌的代谢活性鼠伤寒减少82.2％，胞外蛋白，胞外多糖和胞外DNA分别从68.5降低至12.3μg/ mL，从32.1降低至8.4μg/ mL，从26.​​5降低至8.6μg/ mL qRT-PCR分析表明CNP治疗下降-调节与细胞外蛋白，细胞外多糖和细胞外DNA有关的基因的转录。此外，CNP可以去除禽蛋壳上的鼠伤寒沙门氏菌生物膜，而不会显着影响感官质量。