Brassica is a very important genus of Brassicaceae, including many important oils, vegetables, forage crops, and ornamental horticultural plants. TLP family genes play important regulatory roles in the growth and development of plants. Therefore, this study used a bioinformatics approach to conduct the systematic comparative genomics analysis of TLP gene family in B. napus and other three important Brassicaceae crops. Here, we identified a total of 29 TLP genes from B. napus genome, and they distributed on 16 chromosomes of B. napus. The evolutionary relationship showed that these genes could be divided into six groups from Group A to F. We found that the gene corresponding to Arabidopsis thaliana AT1G43640 was completely lost in B. rapa, B. oleracea and B. napus after whole genome triplication. The gene corresponding to AT1G25280 was retained in all the three species we analysed, belonging to 1:3:6 ratios. Our analyses suggested that there was a selective loss of some genes that might be redundant after genome duplication. This study proposed that the TLP genes in B. napus did not directly expansion compared with its diploid parents B. rapa, and B. oleracea. Instead, an indirect expansion of TLP gene family occurred in its two diploid parents. In addition, the study further utilized RNA-seq to detect the expression pattern of TLP genes between different tissues and two subgenomes. This study systematically conducted the comparative analyses of TLP gene family in B. napus, discussed the loss and expansion of genes after genome duplication. It provided rich gene resources for exploring the molecular mechanism of TLP gene family. Meanwhile, it provided guidance and reference for the research of other gene families in B. napus.

中文翻译：

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甘蓝型油菜全基因组TLP基因家族的鉴定，进化和表达分析

芸苔属芸苔科的一个非常重要的属，包括许多重要的油，蔬菜，饲料作物和观赏性园艺植物。TLP家族基因在植物的生长和发育中起着重要的调节作用。因此，本研究使用生物信息学方法对甘蓝型油菜和其他三种重要十字花科作物的TLP基因家族进行了系统的比较基因组学分析。在这里，我们从甘蓝型油菜的基因组中鉴定出总共29个TLP基因，它们分布在甘蓝型油菜的16个染色体上。进化关系表明，这些基因可以从A组到F组分为6组。我们发现，拟南芥AT1G43640对应的基因在全基因组三倍体分离后完全消失于B. rapa，B。oleracea和B. napus中。在我们分析的所有三个物种中，与AT1G25280相对应的基因均被保留，比例为1：3：6。我们的分析表明，基因组复制后某些基因的选择性丢失可能是多余的。这项研究表明，与其二倍体亲本B. rapa和B. oleracea相比，B。napus中的TLP基因没有直接扩增。相反，在其两个二倍体亲本中发生了TLP基因家族的间接扩增。此外，该研究进一步利用RNA-seq检测TLP基因在不同组织和两个亚基因组之间的表达模式。本研究系统地进行了甘蓝型油菜TLP基因家族的比较分析，讨论了基因组复制后基因的丢失和扩增。为探索TLP基因家族的分子机制提供了丰富的基因资源。与此同时，