Particulate matter (PM) can cause various negative acute and chronic diseases of the respiratory system, including the upper airways. Curcumin has been reported to have anti-inflammatory and anti-oxidative effects; therefore, we investigated the effects of curcumin on nasal fibroblasts exposed to urban PM (UPM). Samples of inferior turbinate tissue were obtained from six patients. Flow cytometry was used to assess the levels of reactive oxygen species (ROS) following the treatment of nasal fibroblasts with UPM and/or curcumin. We evaluated the effects of UPM and/or curcumin on the expression of phosphorylated ERK, Nrf2, HO-1, and SOD2 in fibroblasts by Western blotting. When UPM was applied to nasal fibroblasts, ROS production was significantly increased in a dose-dependent manner. UPM-exposed fibroblasts caused the activation of ERK to increase HO-1 expression and decrease SOD2 expression. Treatment with curcumin reduced the UPM-mediated increase in ROS; this decrease in ROS occurred in a dose-dependent manner. The UPM-induced activation of ERK was inhibited by curcumin. Nrf2 production was also promoted to increase the expression of HO-1 and SOD2 by curcumin. Curcumin reduced ROS production caused by UPM in human nasal fibroblasts in a dose-dependent manner, suggesting that curcumin has anti-oxidative effects and may be useful in the treatment of nasal diseases caused by UPM, such as allergic and chronic rhinitis.

中文翻译：

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姜黄素激活Nrf2 / HO-1途径可抑制暴露于城市颗粒物的人鼻成纤维细胞的氧化应激。

颗粒物（PM）可能引起呼吸系统的各种负面急性和慢性疾病，包括上呼吸道。据报道姜黄素具有抗炎和抗氧化作用。因此，我们研究了姜黄素对暴露于城市PM（UPM）的鼻成纤维细胞的影响。从六名患者中获得下鼻甲组织样品。用UPM和/或姜黄素处理鼻成纤维细胞后，流式细胞仪用于评估活性氧（ROS）的水平。我们通过蛋白质印迹评估了UPM和/或姜黄素对成纤维细胞中磷酸化ERK，Nrf2，HO-1和SOD2表达的影响。当UPM应用于鼻成纤维细胞时，ROS的产生以剂量依赖的方式显着增加。暴露于UPM的成纤维细胞引起ERK活化，从而增加HO-1表达并降低SOD2表达。姜黄素治疗可减少UPM介导的ROS升高；ROS的这种减少以剂量依赖性方式发生。姜黄素可抑制UPM诱导的ERK活化。姜黄素还促进了Nrf2的产生，以增加HO-1和SOD2的表达。姜黄素以剂量依赖性方式减少人鼻成纤维细胞中UPM引起的ROS产生，表明姜黄素具有抗氧化作用，可用于治疗由UPM引起的鼻部疾病，例如过敏性和慢性鼻炎。姜黄素可抑制UPM诱导的ERK活化。姜黄素还促进了Nrf2的产生，以增加HO-1和SOD2的表达。姜黄素以剂量依赖性方式减少人鼻成纤维细胞中UPM引起的ROS产生，表明姜黄素具有抗氧化作用，可用于治疗由UPM引起的鼻部疾病，例如过敏性和慢性鼻炎。姜黄素可抑制UPM诱导的ERK活化。姜黄素还促进了Nrf2的产生，以增加HO-1和SOD2的表达。姜黄素以剂量依赖性方式减少人鼻成纤维细胞中UPM引起的ROS产生，表明姜黄素具有抗氧化作用，可用于治疗由UPM引起的鼻部疾病，例如过敏性和慢性鼻炎。