Melanoma is the most aggressive skin cancer that derived from pigment cells, accounting for the majority of the skin-cancer-related deaths. Despite great development and evolution have been made in surgery, radiotherapy and adjuvant chemotherapy, the prognosis of melanoma patients exhibited no significant improvement. Long noncoding RNAs (lncRNAs) are frequently dysregulated and involved in the development of cancers. LncRNA AFAP1-AS1 has been explored in various cancers, whereas its role and regulatory mechanism in melanoma are not well understood. The expression of AFAP1-AS1 was detected by qRT-PCR. CCK-8, colony formation, transwell and western blot assays were performed to investigate the biological role of AFAP1-AS1 in melanoma. Male BALB/c nude mice were applied for in vivo experiments. The interaction among AFAP1-AS1, miR-653-5p and RAI14 was investigated by RNA pull down, RIP and luciferase reporter assays. AFAP1-AS1 was highly expressed in melanoma cell lines. Suppression of AFAP1-AS1 impaired cell proliferation, migration, invasion and EMT in melanoma. Moreover, AFAP1-AS1 was a ceRNA of RAI14 by competitively binding with miR-653-5p. Besides, miR-653-5p overexpression or RAI14 inhibition could repress tumor growth. Eventually, rescue assays indicated that the function of AFAP1-AS1 in the cellular process of melanoma was dependent on miR-653-5p and RAI14. AFAP1-AS1 exerts its oncogenic function in melanoma by targeting miR-653-5p/RAI14 axis.

中文翻译：

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长的非编码RNA AFAP1-AS1通过靶向miR-653-5p / RAI14轴来加速黑色素瘤的进展

黑色素瘤是起源于色素细胞的最具侵略性的皮肤癌，占皮肤癌相关死亡的大部分。尽管在外科手术，放射疗法和辅助化学疗法方面已经取得了很大的发展和发展，但是黑色素瘤患者的预后并未显示出明显的改善。长的非编码RNA（lncRNA）经常失调，并参与癌症的发展。LncRNA AFAP1-AS1已在多种癌症中得到了探索，但是其在黑素瘤中的作用和调控机制尚不清楚。通过qRT-PCR检测AFAP1-AS1的表达。进行了CCK-8，集落形成，transwell和western印迹分析，以研究AFAP1-AS1在黑色素瘤中的生物学作用。将雄性BALB / c裸鼠用于体内实验。AFAP1-AS1之间的交互，miR-653-5p和RAI14通过RNA下拉，RIP和荧光素酶报告基因分析进行了研究。AFAP1-AS1在黑色素瘤细胞系中高度表达。AFAP1-AS1的抑制损害黑色素瘤中的细胞增殖，迁移，侵袭和EMT。此外，AFAP1-AS1通过与miR-653-5p竞争结合而成为RAI14的ceRNA。此外，miR-653-5p的过度表达或RAI14的抑制可抑制肿瘤的生长。最终，救援分析表明，AFAP1-AS1在黑色素瘤细胞过程中的功能取决于miR-653-5p和RAI14。AFAP1-AS1通过靶向miR-653-5p / RAI14轴在黑色素瘤中发挥其致癌作用。此外，AFAP1-AS1通过与miR-653-5p竞争结合而成为RAI14的ceRNA。此外，miR-653-5p的过度表达或RAI14的抑制可抑制肿瘤的生长。最终，救援分析表明，AFAP1-AS1在黑色素瘤细胞过程中的功能取决于miR-653-5p和RAI14。AFAP1-AS1通过靶向miR-653-5p / RAI14轴在黑色素瘤中发挥其致癌作用。此外，AFAP1-AS1通过与miR-653-5p竞争结合而成为RAI14的ceRNA。此外，miR-653-5p的过度表达或RAI14的抑制可抑制肿瘤的生长。最终，救援分析表明，AFAP1-AS1在黑色素瘤细胞过程中的功能取决于miR-653-5p和RAI14。AFAP1-AS1通过靶向miR-653-5p / RAI14轴在黑色素瘤中发挥其致癌作用。