Abstract

Exposure to lead (Pb) is a major risk factor in reproductive toxicity, somatic, and germ cell genotoxicity. Exposure via deteriorating Pb paints and contaminated air, soil, and water had been the primary routes. However, with increasing reports of Pb accumulation in mushrooms and other food items may increase the etiology of Pb poisoning. The study herein investigated somatic genotoxicity and reproductive abnormalities in mice fed extracts of Pb-contaminated Pleurotus ostreatus. Male mice were fed aqueous extracts of P. ostreatus cultivated in 0, 10, 20, 100, 200, 500, and 1000 mg/L of Pb-contaminated rice straw for 35 days. Testes were analyzed for Pb accumulation, histopathology and relative weight gain, caudal epididymis for abnormal sperm morphology, and bone marrow for micronucleus test. Concentration-related significant increase in Pb accumulation was observed in P. ostreatus and testes of exposed mice. Decrease testicular weight, congestion of blood vessels, necrosis, and disorganization of the seminiferous tubules were observed in treated mice. In addition, fold increase of 2.78, 3.39, 6.67, 7.21, 9.63, and 9.70 in abnormal sperm morphology in accordance with the Pb concentrations respectively, confirmed reproductive toxicity. Significant increase in micronucleated polychromatic (PCE) and normochromatic (NCE) erythrocytes and concentration-related decrease PCE-NCE ratio in the bone marrow of treated mice suggest genome instability. Pb-contaminated P. ostreatus increased somatic and germ cell genotoxicity in mice. This may predispose the mice to genetic related syndromes and reproductive syndromes. It further suggests caution in the consumption of metal laden wild mushrooms and crop plants.

中文翻译：

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饲喂铅污染平菇（白腐真菌）提取物的雄性小家鼠的体细胞和生殖细胞遗传毒性的实验模拟。

摘要

铅（Pb）是生殖毒性，体细胞和生殖细胞遗传毒性的主要危险因素。主要途径是使Pb涂料变质以及污染的空气，土壤和水进行暴露。然而，随着蘑菇和其他食品中铅积累的报道增加，可能会增加铅中毒的病因。本文的研究调查了饲喂受Pb污染的平菇提取物的小鼠的体细胞遗传毒性和生殖异常。雄性小鼠饲喂平菇的水提取物在0、10、20、100、200、500和1000 mg / L的受Pb污染的稻草中培养35天。分析睾丸中的Pb积累，组织病理学和相对体重增加，尾部附睾的精子形态异常以及骨髓的微核试验。在P. ostreatus中观察到与铅相关的铅累积显着增加和裸露小鼠的睾丸。在治疗的小鼠中观察到睾丸重量减少，血管充血，坏死和曲细精管紊乱。另外，根据Pb浓度，异常精子形态分别增加2.78、3.39、6.67、7.21、9.63和9.70倍，证实具有生殖毒性。在治疗小鼠的骨髓中，微核多色（PCE）和常色（NCE）红细胞显着增加，而浓度相关的PCE-NCE比率降低则表明基因组不稳定。铅污染的平菇增加了小鼠的体细胞和生殖细胞遗传毒性。这可能使小鼠易患遗传相关综合症和生殖综合症。它进一步建议在食用金属负载的野生蘑菇和农作物时要谨慎。