Given the continued high prevalence of mosquito-transmitted diseases there is a clear need to develop novel disease and vector control strategies. Biopesticides of microbial origin represent a promising source of new approaches to target disease transmitting mosquito populations. Here we describe the development and characterization of a novel mosquito biopesticide, derived from an air-dried, non-live preparation of the bacterium Chromobacterium sp. Panama (Family: Neisseriaceae). This preparation rapidly and effectively kills the larvae of prominent mosquito vectors, including the dengue and Zika vector Aedes aegypti, and the human malaria vector Anopheles gambiae. During semi-field trials in Puerto Rico, we observed high efficacy of the biopesticide against field-derived Ae. aegypti populations, and against Ae. aegypti and Culex spp. larvae in natural breeding water, indicating the suitability of the biopesticide for use under more natural conditions. In addition to high efficacy, the non-live Csp_P biopesticide has a low effective dose, a long shelf life, high heat stability, and can be incorporated into attractive larval baits, all of which are desirable characteristics for a biopesticide.

Importance We have developed a novel preparation to kill mosquitoes from an abundant soil bacterium, Chromobacterium sp. Panama. This preparation is an air-dried powder containing no live bacteria, which can be incorporated into an attractive bait and fed directly to mosquito larvae. We demonstrate that the preparation has broad spectrum activity against the larval form of the mosquitoes responsible for the transmission of malaria and the dengue, chikungunya, Yellow Fever, West Nile and Zika viruses, as well as mosquito larvae that are already resistant to commonly used mosquitocidal chemicals. Our preparation possesses many favourable traits: it kills at a low dosage, and does not lose activity when exposed to high temperatures, all of which suggest it could eventually become an effective new tool for controlling mosquitoes and the diseases they spread.

鉴于蚊子传播疾病的持续高流行，显然需要开发新的疾病和病媒控制策略。微生物来源的生物农药代表了靶向疾病传播蚊子种群的新方法的有希望的来源。在这里，我们描述了一种新型的灭蚊生物杀虫剂的开发和特性，该灭活剂是从细菌Chromobacterium sp。的风干，非活体制剂中获得的。巴拿马（家庭：奈瑟菌科）。该制剂可快速有效地杀死主要的蚊媒幼虫，包括登革热和寨卡病毒伊蚊，人类疟疾冈比亚按蚊。在波多黎各的半田间试验中，我们观察到该生物农药对田间衍生的Ae具有很高的功效。埃及人，反对Ae。aegypti和Culex spp。天然繁殖水中的幼虫，表明该生物农药适合在更自然的条件下使用。除高功效外，非活性Csp_P生物农药具有低有效剂量，长保质期，高热稳定性，并且可以掺入诱人的幼虫诱饵中，所有这些都是生物农药所需的特性。

重要性我们开发了一种新颖的制剂，可以杀死丰富的土壤细菌色杆菌中的蚊子sp。巴拿马。该制剂是不含活细菌的风干粉末，可将其掺入诱饵中并直接喂入蚊虫幼虫。我们证明该制剂对负责传播疟疾和登革热，基孔肯雅热，黄热病，西尼罗河病毒和寨卡病毒的蚊子的幼虫形式具有广谱活性，以及​​已经对常用蚊虫具有抗性的蚊子幼虫化学药品。我们的制剂具有许多有利的特性：以低剂量杀死，暴露于高温下也不会失去活性，所有这些都表明它最终可能成为控制蚊子及其传播疾病的有效新工具。