The anti-aging activities on Caenorhabditis elegans of a polysaccharide, O-acetyl glucomannan (LPR), purified from roots of Lilium davidii var. unicolor Cotton, were assessed by observing the mean lifespan, reproduction, pharyngeal pumping and stress response on nematodes. Additionally, the fluorescence intensity of lipofuscin and the level of reactive oxygen species (ROS) were detected. Also the activities of superoxide dismutase (SOD), catalase (CAT) and contents of malondialdehyde (MDA) were determined by the kit method. The results showed that LPR effectively delayed the aging of C. elegans in a dose-dependent manner. When the concentration reached 4 mg/mL, LPR extended the mean lifespan of C. elegans by up to 40%, 61% (P < 0.01) and 50% (P < 0.05) under normal, thermal and oxidative stress culture conditions, respectively. Moreover, LPR remarkably increased the reproduction duration of the nematodes at a concentration of 1 mg/L, and significantly decreased the ROS and lipofuscin level of C. elegans in three dosage groups. Further study illustrated that LPR at 4 mg/mL strongly increased the activity of SOD and CAT by 39.03% (P < 0.01) and 41.89% (P < 0.05), and decreased the lipid peroxidation of MDA level in C. elegans by 52.59% (P < 0.005) compared to a control. It was inferred that LPR provided stress resistance to heat and oxidation, and prolonged the lifespan of wild type N2 C. elegans mainly by elevating the function of nematode antioxidant defense systems and by scavenging free radicals. These findings provided evidence for the anti-aging properties of this polysaccharide from L. davidii.

中文翻译：

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百合百合根的多糖O-乙酰葡甘露聚糖对秀丽隐杆线虫的抗衰老作用。单色棉。

从百合百合根提取的多糖O-乙酰葡甘露聚糖（LPR）对秀丽隐杆线虫的抗衰老活性。通过观察平均寿命，繁殖，咽抽动和对线虫的应激反应来评估单色棉。另外，还检测了脂褐素的荧光强度和活性氧（ROS）水平。还通过试剂盒法测定了超氧化物歧化酶（SOD），过氧化氢酶（CAT）的活性和丙二醛（MDA）的含量。结果表明，LPR以剂量依赖性方式有效地延迟了秀丽隐杆线虫的衰老。当浓度达到4 mg / mL时，LPR在正常，热和氧化应激培养条件下，线虫的平均寿命分别延长40％，61％（P <0.01）和50％（P <0.05）。 。此外，LPR在1 mg / L的浓度下显着增加了线虫的繁殖持续时间，并在三个剂量组中显着降低了秀丽隐杆线虫的ROS和脂褐素水平。进一步的研究表明，在4 mg / mL的LPR下，线虫的SOD和CAT活性显着增加39.03％（P <0.01）和41.89％（P <0.05），线虫中MDA脂质过氧化作用降低52.59％ （P <0.005）与对照相比。可以推断，LPR主要通过增强线虫抗氧化防御系统的功能和清除自由基来提供抗热和抗氧化的能力，并延长了野生型线虫的寿命。这些发现为来自大卫氏乳杆菌的这种多糖的抗衰老特性提供了证据。