Intracellular trafficking requires extensive changes in membrane morphology. Cells use several distinct molecular factors and physical cues to remodel membranes. Here, we highlight recent advances in identifying the biophysical mechanisms of membrane curvature generation. In particular, we focus on the cooperation of molecular and physical drivers of membrane bending during three stages of vesiculation: budding, cargo selection, and scission. Taken together, the studies reviewed here emphasize that, rather than a single dominant mechanism, several mechanisms typically work in parallel during each step of membrane remodeling. Important challenges for the future of this field are to understand how multiple mechanisms work together synergistically and how a series of stochastic events can be combined to achieve a deterministic result-assembly of the trafficking vesicle.

中文翻译：

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膜弯曲和交通中的生物物理力。

细胞内运输需要膜形态的广泛变化。细胞使用几种不同的分子因子和物理线索来重塑细胞膜。在这里，我们重点介绍了确定膜曲率生成的生物物理机制的最新进展。特别是，我们专注于在囊泡形成的三个阶段中膜弯曲的分子和物理驱动因素的合作：萌芽、货物选择和断裂。总之，这里审查的研究强调，在膜重塑的每个步骤中，通常有几种机制并行工作，而不是单一的主导机制。