Hypochlorous acid (HClO) as a reactive oxygen species (ROS) played an important role in many pathological and physiological processes. HClO could kill invading pathogens and bacteria in the immune system. But excessive concentrations of HClO may cause many diseases. Near-infrared fluorescent probes have strong tissue penetrating ability, low autofluorescence interference, and broad application prospects in the field of fluorescence imaging research. In this work, we synthesized a new near-infrared (670 nm) “dual-mode” fluorescent dye NSSN based on the benzothiazole framework for the visual detection of HClO. The NSSN had a large Stokes shift (220 nm), good selectivity, ratiometric near-infrared and low detection limit (0.13 μM). In addition, NSSN could achieve mitochondrial target detection of HClO in HeLa cells, also HClO could be visually detected in nude mice by NSSN. Thus NSSN is expected to be used in the early diagnosis of HClO.

次氯酸（HClO）作为活性氧（ROS）在许多病理和生理过程中起着重要作用。HClO可以杀死免疫系统中入侵的病原体和细菌。但是过量的HClO可能会导致许多疾病。近红外荧光探针具有较强的组织穿透能力，自发荧光干扰小，在荧光成像研究领域具有广阔的应用前景。在这项工作中，我们基于苯并噻唑框架合成了一种新的近红外（670 nm）“双模”荧光染料NSSN，用于视觉检测HClO。所述NSSN有一个大的斯托克斯频移（220纳米），良好的选择性，比率近红外和低检测限（0.13μM）。另外，NSSN它可以实现HeLa细胞中HClO的线粒体靶标检测，也可以通过NSSN在裸鼠中目测HClO 。因此，有望将NSSN用于HClO的早期诊断。