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Investigation and comparison of the protective activities of three functional proteins-lactoferrin, α-lactalbumin, and β-lactoglobulin-in cerebral ischemia reperfusion injury.
Journal of Dairy Science ( IF 3.5 ) Pub Date : 2020-03-27 , DOI: 10.3168/jds.2019-17725
Hui-Ying Li 1 , Peng Li 2 , Huai-Gu Yang 1 , Qian-Qian Yao 1 , Sheng-Nan Huang 1 , Jia-Qi Wang 1 , Nan Zheng 1
Affiliation  

The objective of this study was to evaluate the protection conferred by lactoferrin, α-lactalbumin, and β-lactoglobulin in cerebral ischemia reperfusion (I/R) injury. Rat pheochromocytoma (PC12) cells were used to construct an oxygen and glucose deprivation model in vitro, and ICR mice underwent carotid artery "ligation-relaxation" to construct a cerebral I/R injury model in vivo. The levels of toll-like receptor 4 (TLR4) and downstream factors including nuclear factor-κB, tumor necrosis factor-α, and IL-1β were measured. Metabonomics detection and data mining were conducted to identify the specific metabolic sponsor of the 3 proteins. The results showed that lactoferrin, α-lactalbumin, and β-lactoglobulin protected neurons from cerebral I/R injury by increasing the level of bopindolol and subsequently inhibiting the TLR4-related pathway to different degrees; β-lactoglobulin had the strongest activity of the 3 proteins. In summary, this study is the first to investigate and compare the protective effects of lactoferrin, α-lactalbumin, and β-lactoglobulin in a cerebral stroke model. The results implicate TLR4 as a novel target of the 3 bioactive proteins to prevent cerebral I/R injury.

中文翻译:

三种功能蛋白-乳铁蛋白,α-乳白蛋白和β-乳球蛋白在脑缺血再灌注损伤中的保护活性研究与比较。

这项研究的目的是评估乳铁蛋白,α-乳白蛋白和β-乳球蛋白对脑缺血再灌注(I / R)损伤的保护作用。大鼠嗜铬细胞瘤(PC12)细胞用于体外建立氧和葡萄糖剥夺模型,对ICR小鼠进行颈动脉“结扎松弛”以体内建立脑I / R损伤模型。测量了toll样受体4(TLR4)和下游因子的水平,包括核因子-κB,肿瘤坏死因子-α和IL-1β。进行了代谢组学检测和数据挖掘,以鉴定这3种蛋白的特定代谢赞助者。结果表明,乳铁蛋白,α-乳白蛋白,β-乳球蛋白通过增加波潘多洛的水平并随后不同程度地抑制TLR4相关途径来保护神经元免受脑I / R损伤;β-乳球蛋白具有3种蛋白质中最强的活性。总之,该研究是第一个研究和比较乳铁蛋白,α-乳白蛋白和β-乳球蛋白在脑卒中模型中的保护作用。结果暗示TLR4作为3种生物活性蛋白的新靶标,可预防脑I / R损伤。
更新日期:2020-03-27
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