An automated polystyrene-divinyl benzene (PS-DVB) based standard preloaded-pipet tip-SPE method was developed for the prompt and precise quantification of contaminants in plant extracts using the example of polycyclic aromatic hydrocarbons (PAHs). The objective of the technique was to establish a high-throughput SPE-automated workflow with minimized personal exposure to tremendously carcinogenic PAHs during standard and sample preparation. HPLC-FLD was used for the analysis of PAHs. LOD and LOQ for the investigated PAHs were found to be 16 ppb and 59 ppb, respectively. The recoveries were between 90–102% (RSD < 8%) for the standard compounds in the pure solvent, and between 90–95% (RSD < 10%) for spiked black tea extracts. A contaminated Primulae flos extract was also subjected to the preloaded PhyTip®-SPE scheme and revealed 70.8 μg kg⁻¹ chrysene and 60.9 μg kg⁻¹ benzo(a)anthracene. In order to enhance method reproducibility and to increase the sample throughput, a PhyNexus® (San Jose, CA), MEA™ Robotic Purification System was used for the automation of the SPE method. The automated method with a duration of 17 minutes for 12 samples revealed high recoveries and reproducibility for the PAH standards (90–103% with RSD < 8%) and between 90–95% (RSD < 10%) for spiked black tea extracts. The presented analytical scheme provides an excellent tool for rapid and precise determination of PAHs from complex mixtures.

中文翻译：

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自动预装移液器吸头SPE方法可准确定量茶叶中的致癌多环芳烃

开发了一种基于聚苯乙烯-二乙烯基苯（PS-DVB）的自动标准预装吸头-SPE方法，以多环芳烃（PAH）为例，快速，准确地定量植物提取物中的污染物。该技术的目的是建立一种高通量的SPE自动化工作流程，并在标准和样品制备过程中将个人对巨大致癌PAH的暴露降至最低。HPLC-FLD用于分析PAHs。研究的PAH的LOD和LOQ分别为16 ppb和59 ppb。在纯溶剂中标准化合物的回收率在90–102％（RSD <8％）之间，而加标红茶提取物的回收率在90–95％（RSD <10％）之间。污染的报春花提取物也进行了预加载PhyTip®-SPE方案和显示70.8微克千克^-1屈和60.9微克千克^-1苯并（一）蒽。为了提高方法的可重复性并增加样品通量，使用了PhyNexus？（美国加利福尼亚州圣何塞）的MEA™机器人纯化系统来实现SPE方法的自动化。自动化方法的时间为12分钟，采样时间为17分钟，显示出PAH标准品的回收率和重现性较高（RSD <8％时为90–103％，加标的红茶提取物为90–95％（RSD <10％）。提出的分析方案为从复杂混合物中快速准确测定多环芳烃提供了极好的工具。