Collagen hydrogels have been widely investigated as scaffolds for tissue engineering due to their biocompatibility and capacity to promote cell adhesion. However, insufficient mechanical strength and rapid degradation properties remain the major obstacles for their applications. In the present study, type-I tilapia collagen (TC) was functionalized to form methacrylated tilapia collagen (MATC) by introducing methacrylic acid, developing a photo-cross-linked PEGDA-MATC hydrogel. The mechanical strength of PEGDA-MATC hydrogel could be tuned by adjusting the pH of the precursor solutions, which was decreased with the pH increased. At a pH 5 condition, PEGDA-MATC showed the highest compressive fracture stress (1.31 MPa). Compared to the PEGDA-TC hydrogel, PEGDA-MATC hydrogel exhibited similar swelling behavior to PEGDA-TC hydrogel in PBS solutions, but higher residual mass ratio (PEGDA-MATC, 213.2 ± 2.8%) than PEGDA-TC hydrogel (199.4 ± 3.8%) when cultured with type-I collagenase. PEGDA-MATC hydrogel showed sustained BSA release capacity for 6 days, and the BSA release ratio was significantly (p < 0.05) decreased with increasing concentration of loaded-BSA (68.6% at 4 mg mL-1, 42.2% at 8 mg mL-1). The PEGDA-MATC hydrogel allowed cell adhesion and proliferation in vitro. These results demonstrated that PEGDA-MATC hydrogel might be a potential scaffold for tissue engineering applications.

中文翻译：

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光交联的功能化I型胶原蛋白（Oreochromis niloticus）和聚乙二醇二丙烯酸酯水凝胶的开发和表征。

胶原蛋白水凝胶由于其生物相容性和促进细胞粘附的能力而被广泛研究为组织工程的支架。然而，机械强度不足和快速降解性能仍然是其应用的主要障碍。在本研究中，通过引入甲基丙烯酸功能化I型罗非鱼胶原蛋白（TC）以形成甲基丙烯酸罗非鱼胶原蛋白（MATC），从而开发出光交联的PEGDA-MATC水凝胶。PEGDA-MATC水凝胶的机械强度可以通过调节前体溶液的pH值来调节，该pH值随pH值的升高而降低。在pH 5条件下，PEGDA-MATC表现出最高的压缩断裂应力（1.31 MPa）。与PEGDA-TC水凝胶相比，PEGDA-MATC水凝胶在PBS溶液中表现出与PEGDA-TC水凝胶相似的溶胀行为，但使用I型胶原酶培养时，残留质量比（PEGDA-MATC，213.2±2.8％）比PEGDA-TC水凝胶（199.4±3.8％）高。PEGDA-MATC水凝胶显示持续6天的BSA释放能力，并且BSA释放率随负载的BSA浓度的增加而显着降低（p <0.05）（4 mg mL-1为68.6％，8 mg mL-为42.2％ 1）。PEGDA-MATC水凝胶允许细胞在体外粘附和增殖。这些结果表明，PEGDA-MATC水凝胶可能是组织工程应用的潜在支架。05）随负载的BSA浓度的增加而降低（4 mg mL-1时为68.6％，8 mg mL-1时为42.2％）。PEGDA-MATC水凝胶允许细胞在体外粘附和增殖。这些结果表明，PEGDA-MATC水凝胶可能是组织工程应用的潜在支架。05）随负载的BSA浓度的增加而降低（4 mg mL-1时为68.6％，8 mg mL-1时为42.2％）。PEGDA-MATC水凝胶允许细胞在体外粘附和增殖。这些结果表明，PEGDA-MATC水凝胶可能是组织工程应用的潜在支架。