The use of orchids in herbal medicine has a very long history. Dendrobium species are known to produce a variety of secondary metabolites such as phenanthrens, bibenzyls, fluorenones and sesquiterpenes, and alkaloids and are responsible for their wide variety of medicinal properties. For decades, bibenzyls, which are the main bioactive components derived from Dendrobium species, have been subjected to extensive investigation as likely candidates for cancer treatment. The present study was undertaken to investigate the effect of moscatilin, a bibenzyl derivative from the orchid Dendrobium loddigesii on human melanoma cells. In A375 cells compound moscatilin showed a clear dose-response relationship in the range of 6.25-50 μM concentrations. In addition, we demonstrated an apoptotic response after treatment of cancer cells with this bibenzyl compound at 6.25 and 12.5 μM concentrations that probably involves PTEN activity, inhibition of Hsp70 expression and reactive oxygen species production. Alternatively, the inhibition of the caspase cascade at higher concentrations, 25 and 50 μM, correlated with additional reactive oxygen species increase, probably switched the mode of moscatilin-induced cell death from apoptosis to necrosis.

中文翻译：

---

Moscatilin是来自兰花石derivative兰的双苄基衍生物，可诱导黑色素瘤细胞凋亡。

在草药中使用兰花已有很长的历史。已知铁皮石end种会产生多种次级代谢产物，例如菲，联苄，芴酮和倍半萜以及生物碱，并对其广泛的药用特性负责。数十年来，作为石s物种的主要生物活性成分的联苄已作为癌症治疗的可能候选物进行了广泛研究。进行本研究以研究莫斯卡蒂林（一种来自兰花石end的联苯苄衍生物）对人黑素瘤细胞的作用。在A375细胞中，化合物Moscatilin在6.25-50μM浓度范围内表现出明显的剂量反应关系。此外，我们在6.25和12.5μM浓度的联苯苄基化合物处理癌细胞后证明了凋亡反应，可能涉及PTEN活性，Hsp70表达抑制和活性氧的产生。或者，在更高浓度（25和50μM）下对caspase级联的抑制与其他活性氧的增加相关，可能将莫卡替林诱导的细胞死亡方式从凋亡转变为坏死。