Developing new probes for visualizing H₂S in the endoplasmic reticulum (ER) is of great significance in physiological and pathological fields in that the probe can antagonise ER stress. Herein, we try to explore efficient reporting fluorophores based on three pyrimidine derivatives (L1, L2 and L3), and eventually, a novel probe WH2S was fabricated by using emissive pyrimidine derivative (L1) as the reporting fluorophore. Upon the addition of H₂S, the probe processed a thiolytic cleavage to regenerate L1, delivering a remarkable fluorescence enhancement. Probe WH2S presents a perfect selectivity, high sensitivity and low detection limit (3.81 μM) towards H₂S in the buffer media. Most importantly, fluorescence microscopy experiments demonstrate that WH2S can precisely accumulate in ER and detect exogenous/endogenous H₂S at cellular level. These results imply that probe WH2S possesses great potentiality in tracking target H₂S in complicated and changeable living systems.

开发新的可视化内质网（ER）中H ₂ S的探针在生理和病理学领域具有重要意义，因为该探针可以拮抗ER应激。在本文中，我们尝试探索基于三种嘧啶衍生物（L1，L2和L3）的有效报告荧光团，最终，使用发射嘧啶衍生物（L1）作为报告荧光团制备了新型探针WH2S。添加H ₂ S后，该探针进行了硫解裂解以再生L1，从而显着增强了荧光。探头WH2S对缓冲液中的H ₂ S具有完美的选择性，高灵敏度和低检测限（3.81μM）。最重要的是，荧光显微镜实验表明，WH2S可正是在ER积累和检测外源性/内源性^ h ₂ S中细胞水平。这些结果表明，探针WH2S在复杂多变的生命系统中具有追踪目标H ₂ S的巨大潜力。