Di-n-butyl phthalate, one of the most easily detected pollutants of phthalate esters in the environment, has been added to the priority list of hazardous substances by many countries. As one of low molecular weight phthalates, Di-n-butyl phthalate may have a great adverse potency on various aquatic organisms. In this study, the juvenile red snapper, Lutjanus argentimaculatus, was exposed to the concentrations of Di-n-butyl phthalate (20 μg L-1, 100 μg L-1 and 500 μg L-1) for 15 days. EROD activities and CYP1A levels were measured in liver and gill tissues. In gills, the similar effect has been found to inhibit or induce EROD activities and CYP1A levels, and there existed a good correlation between them. Whereas in the case of the liver, a moderate correlation was observed between EROD activities and CYP1A levels, which was mainly due to the inhibited EROD activities and the CYP1A levels with no significant difference by day 15. In conclusion, this study revealed the similar and different effects of cytochrome P450 enzymes on fish in the time-, concentration-, and tissue-dependent Di-n-butyl phthalate exposure. Furthermore, as the adverse effects indicated between CYP1A levels and EROD activities, metabolic mechanisms of phthalates in different tissues should be highly emphasized in future studies.

中文翻译：

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邻苯二甲酸二正丁酯对幼年红鲷鱼（Lutjanus argentimaculatus）的g和肝特异性EROD活性和CYP1A水平的影响。

邻苯二甲酸二正丁酯是环境中最容易检测到的邻苯二甲酸酯类污染物之一，已被许多国家列入危险物质的优先清单。作为低分子量邻苯二甲酸酯之一，邻苯二甲酸二正丁酯可能对各种水生生物具有很大的不利影响。在这项研究中，将幼年红鲷鱼Lutjanus argentimaculatus暴露于邻苯二甲酸二正丁酯（20μgL-1、100μgL-1和500μgL-1）的浓度15天。在肝脏和g组织中测量EROD活性和CYP1A水平。在g中，已发现抑制或诱导EROD活性和CYP1A水平具有相似的作用，并且它们之间存在良好的相关性。肝脏方面，EROD活性与CYP1A水平呈中等程度的相关性，这主要是由于第15天EROD活性和CYP1A水平受到抑制，在第15天时无显着差异。总之，这项研究揭示了细胞色素P450酶对鱼类的时间，浓度和组织依赖性具有相似和不同的作用邻苯二甲酸二正丁酯暴露。此外，由于CYP1A水平与EROD活性之间存在不良反应，因此在今后的研究中应高度重视邻苯二甲酸酯在不同组织中的代谢机制。