ABSTRACT In this study, we produced a specific monoclonal antibody (mAb) against triadimefon (TDF) and triadimenol (TDN). The half-maximal inhibitory concentration for TDF and TDN was 5.25 and 9.98 ng/mL, respectively, with a linear detection range (IC20–IC80) of 1.09–25.35 and 2.29–43.61 ng/mL, respectively. Subsequently, we developed a colloidal gold immunochromatography test strip for the analysis of TDF and TDN in tomato. The results obtained from the colloidal gold immunochromatography test strip were in accordance with those obtained from the indirect competitive enzyme-linked immunosorbent assay. The colloidal gold immunochromatography test strip is efficient and suitable for on-site detection of TDF and TDN.

中文翻译：

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一种基于单克隆抗体的胶体金免疫层析试纸条，用于快速检测食品中的三唑酮和三唑醇

摘要 在本研究中，我们生产了一种针对三唑酮 (TDF) 和三唑醇 (TDN) 的特异性单克隆抗体 (mAb)。TDF 和 TDN 的半数最大抑制浓度分别为 5.25 和 9.98 ng/mL，线性检测范围 (IC20-IC80) 分别为 1.09-25.35 和 2.29-43.61 ng/mL。随后，我们开发了一种胶体金免疫层析试纸条，用于分析番茄中的 TDF 和 TDN。胶体金免疫层析试纸条结果与间接竞争酶联免疫吸附试验结果一致。胶体金免疫层析试纸条高效，适用于现场检测TDF和TDN。