Abstract A new porous metal-organic framework material, {Ca3(TATAB)2 (H2O)(MeOH)}(DMF)3}n (1, TATAB3- = 4,4′,4′′-s-triazine-1,3,5-triyltri-p-aminobenzoate), with considerable biocompatibility was synthesized by reaction of the Ca(NO3)2 and the H3TATAB ligand in DMF/MeOH solution. Nanostructure 1 can also be prepared by sonochemical process at ambient temperature. Nitrogen adsorption measurements revealed the presence of micropores as well as moderately high BET surface areas in the activated nanostructured 1 (1a). The incorporation of the drug 5-fluorouracil (5-FU) into 1a was 38.6 wt% per gram of 1a. 5-FU is released in a highly controlled and progressive fashion with 88.7% of the drug released after 72 h. The CCK8 assay was performed to evaluate the inhibitory effect of 5-FU@1a on NCI-H292 and NCI-H460 human lung cancer cells, and the transwell assay was conducted to observe the migration and invasion ability of cancer cells after 5-FU@1a treatment. The Annexin V-FITC/PI assay was carried out to evaluate the level of cancer cell apoptosis. The results above indicate 5-FU@1a has excellent anticancer activity in vitro. The in vivo xenograft model was applied, and the results suggested 5-FU@1a could reduce the volume and the weight of the tumor in vivo. Graphical Abstract

中文翻译：

---

超声辅助合成新型纳米结构 Ca(II)-MOF 作为 5-FU 递送系统抑制人肺癌细胞增殖、迁移、侵袭并诱导细胞凋亡

摘要 一种新型多孔金属有机骨架材料，{Ca3(TATAB)2 (H2O)(MeOH)}(DMF)3}n (1, TATAB3- = 4,4',4''-s-triazine-1, 3,5-triyltri-p-aminobenzoate)，具有相当大的生物相容性是通过 Ca(NO3)2 和 H3TATAB 配体在 DMF/MeOH 溶液中的反应合成的。纳米结构 1 也可以在环境温度下通过声化学过程制备。氮吸附测量显示在活化的纳米结构 1 (1a) 中存在微孔以及中等高的 BET 表面积。药物 5-氟尿嘧啶 (5-FU) 在 1a 中的掺入量为 38.6 wt%/g 1a。5-FU 以高度受控和渐进的方式释放，72 小时后释放 88.7% 的药物。进行CCK8测定以评估5-FU@1a对NCI-H292和NCI-H460人肺癌细胞的抑制作用，并通过Transwell实验观察5-FU@1a处理后癌细胞的迁移和侵袭能力。进行Annexin V-FITC/PI测定以评估癌细胞凋亡水平。以上结果表明5-FU@1a在体外具有优异的抗癌活性。应用体内异种移植模型，结果表明5-FU@1a可以减少体内肿瘤的体积和重量。图形概要 结果表明5-FU@1a可以减少体内肿瘤的体积和重量。图形概要 结果表明5-FU@1a可以减少体内肿瘤的体积和重量。图形概要