Yeast Snf1 (Sucrose non-fermenting1), mammalian AMPK (5′ AMP-activated protein kinase) and plant SnRK1 (Snf1-Related Kinase1) are conserved heterotrimeric kinase complexes that re-establish energy homeostasis following stress. The hormone abscisic acid (ABA) plays a crucial role in plant stress response. Activation of SnRK1 or ABA signaling results in overlapping transcriptional changes, suggesting these stress pathways share common targets. To investigate how SnRK1 and ABA interact during stress response in Arabidopsis thaliana, we screened the SnRK1 complex by yeast two-hybrid against a library of proteins encoded by 258 ABA-regulated genes. Here, we identify 125 SnRK1- interacting proteins (SnIPs). Network analysis indicates that a subset of SnIPs form signaling modules in response to abiotic stress. Functional studies show the involvement of SnRK1 and select SnIPs in abiotic stress responses. This targeted study uncovers the largest set of SnRK1 interactors, which can be used to further characterize SnRK1 role in plant survival under stress.

酵母Snf1（蔗糖非发酵1），哺乳动物AMPK（5'AMP激活的蛋白激酶）和植物SnRK1（Snf1相关激酶1）是保守的异三聚体激酶复合物，可在压力后重新建立能量稳态。脱落酸（ABA）在植物胁迫反应中起关键作用。SnRK1或ABA信号传导的激活导致重叠的转录变化，表明这些应激途径共有共同的靶标。调查SnRK1和ABA在拟南芥胁迫响应过程中如何相互作用。，我们针对两种由258种ABA调控基因编码的蛋白质文库，通过酵母双杂交筛选了SnRK1复合物。在这里，我们确定了125个SnRK1相互作用蛋白（SnIP）。网络分析表明，SnIP的一个子集可响应非生物胁迫形成信号传导模块。功能研究表明，SnRK1和选择的SnIPs参与非生物胁迫反应。这项有针对性的研究揭示了最大的SnRK1相互作用体集，可用于进一步表征SnRK1在胁迫下植物存活中的作用。