TBI is the main cause of death and disability in individuals aged 1-45 in Western countries. One of the main challenges of TBI at present is the lack of specific diagnostic biomarkers, especially for mild TBI (mTBI), which remains currently difficult to value in clinical practice. In this context MiRNAs may be important mediators of the profound molecular and cellular changes that occur after TBI in both the short and the long term. Recently, plasma miRNAs profiling in human TBI, have revealed dynamic temporal regulation of miRNA expression within the cortex. Aim of this study was to select a specific miRNAs panel for mTBI, by focusing the research on the prognostic meaning of miRNAs in the hours following the trauma, in order to be able to use this MIRNAs as potential biomarkers useful for monitoring the follow up of mild TBI. Serum levels of 17 miRNAs were measured by RT-quantitative polymerase chain reaction (qPCR) in 20 patients with mTBI at three different time-points (0 h, 24 h, 48 h) and in 10 controls. For 15 miRNAs we found a significant differences in the comparison among the three time points: for each of these miRNAs the values were greater at baseline and progressively reduced at 24 h and 48 h. These data allow us to consider the miRNAs included in panel as sensitive and specific biomarkers for mTBI, useful in monitoring the post-trauma period.

中文翻译：

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循环miRNAs表达是轻度脑外伤的潜在生物标志物。

在西方国家，TBI是导致1至45岁的人死亡和致残的主要原因。目前，TBI的主要挑战之一是缺乏特异性的诊断生物标记物，特别是对于轻度TBI（mTBI），目前在临床实践中仍然难以对其进行评估。在这种情况下，miRNA可能是短期和长期TBI后发生的深刻分子和细胞变化的重要介质。最近，人TBI中的血浆miRNA分析显示出皮质内miRNA表达的动态时间调控。这项研究的目的是通过针对创伤后数小时内miRNA的预后意义进行研究，从而为mTBI选择一个特定的miRNA组，以便能够将该MIRNA用作可用于监测MRNA随访的潜在生物标志物。轻度TBI。通过RT定量聚合酶链反应（qPCR）在20个mTBI患者的三个不同时间点（0 h，24 h，48 h）和10个对照中测量了17种miRNA的血清水平。对于15个miRNA，我们发现三个时间点之间的比较存在显着差异：对于这些miRNA中的每一个，其值在基线时都较大，在24 h和48 h时逐渐降低。这些数据使我们能够将面板中包含的miRNA视为mTBI的敏感和特异性生物标志物，可用于监测创伤后时期。对于这些miRNA中的每一个，其值在基线时都较大，在24 h和48 h时逐渐降低。这些数据使我们可以将面板中包含的miRNA视为mTBI的敏感和特异性生物标志物，可用于监测创伤后时期。对于这些miRNA中的每一个，其值在基线时都较大，在24 h和48 h时逐渐降低。这些数据使我们可以将面板中包含的miRNA视为mTBI的敏感和特异性生物标志物，可用于监测创伤后时期。