A novel bio-based zein-graft-β-cyclodextrin film was synthesized for cholesterol adsorption at room temperature. Immobilization of β-cyclodextrin (β-CD) to zein was achieved by the Maillard reaction and the zein-graft-β-CD powders showed higher glass transition temperature and higher solubility in water. Zein-graft-β-CD films, prepared by solvent evaporation casting, showed excellent capacity for cholesterol adsorption. The F1:1 has the best adsorption properties, and the maximum adsorption capacity can reach 5.70 ± 0.56 mg cholesterol/g film. The adsorption cholesterol mechanism of zein-graft-β-CD film was correspond to the pseudo-first order kinetic model. In addition, the zein-graft-β-CD film retained an adsorption capacity of 3.10 ± 0.89 mg cholesterol/g film after three reuses. Using composite enzyme (1 mg/mL papain and 1 mg/mL amylase), the film (F1:1) degradation reached 98.81% in 1 week. These results suggest that zein-graft-β-CD film has potential as a nature-based adsorbent for cholesterol adsorption and could be used in the food industry.

中文翻译：

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可生物降解的玉米醇溶蛋白-β-环糊精膜对胆固醇的吸附去除。

合成了一种新型的基于生物的玉米醇溶蛋白-β-环糊精薄膜，用于室温下胆固醇的吸附。通过美拉德反应将β-环糊精（β-CD）固定到玉米蛋白上，玉米醇溶蛋白接枝的β-CD粉末显示出更高的玻璃化转变温度和更高的水溶性。通过溶剂蒸发流延制备的玉米醇溶蛋白-β-CD膜，表现出优异的胆固醇吸附能力。F1：1具有最佳的吸附性能，最大吸附容量可达到5.70±0.56 mg胆固醇/ g膜。玉米醇溶蛋白-β-CD膜的吸附胆固醇机制与拟一级动力学模型相对应。另外，玉米醇溶蛋白-β-CD膜在三次重复使用后仍保持3.10±0.89 mg胆固醇/ g膜的吸附能力。使用复合酶（1 mg / mL木瓜蛋白酶和1 mg / mL淀粉酶），1周后薄膜（F1：1）的降解率达到98.81％。这些结果表明，玉米醇溶蛋白-β-CD膜具有作为天然的胆固醇吸附剂的潜力，可用于食品工业。