OBJECTIVE Guided bone regeneration (GBR) often involves the use of membranes as barriers for soft tissues. Commercially available membranes, however, do not possess an adequately low degradation rate, resulting in limited barrier function. The purpose of this study was to develop and assess the physicochemical and biological characteristics of a novel poly(l-lactic acid/caprolactone) (PLCL) bilayer membrane and determine its usefulness for GBR application. METHODS The experimental bilayer membrane was prepared via a two-step freezing and lyophilization process with a PLCL solution. Next, the PLCL membrane was investigated regarding tensile strength, surface roughness, in vitro degradation and clinical operability. In addition, cell proliferation and differentiation were investigated on each layer of the experimental membrane. For all experiments, a commercially available poly(lactic-co-glycolic) acid membrane was used as a control. RESULTS In vitro analysis of the PLCL bilayer membrane revealed suitable mechanical strength combined with high breaking strain, which contributed to membrane operability. In addition, the PLCL bilayer membrane had enhanced stability compared to the commercial control due to its slower degradation, and was capable of supporting cell growth and osteogenic differentiation. SIGNIFICANCE The current study confirmed that the PLCL membrane possessed a high biocompatibility and slow degradation rate that contributes to prolonged barrier function and bone regeneration. Altogether, it was considered that the PLCL bilayer membrane developed in this study was applicable for GBR treatment.

中文翻译：

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用于GBR的新型聚乳酸/己内酯双层膜的制备。

目的引导性骨再生（GBR）通常涉及使用膜作为软组织的屏障。然而，市售的膜不具有足够低的降解速率，从而导致有限的屏障功能。这项研究的目的是开发和评估新型聚（l-乳酸/己内酯）（PLCL）双层膜的理化和生物学特性，并确定其在GBR应用中的有用性。方法采用PLCL溶液通过两步冷冻和冻干过程制备实验双层膜。接下来，对PLCL膜进行了拉伸强度，表面粗糙度，体外降解和临床可操作性的研究。另外，在实验膜的每一层上研究了细胞增殖和分化。对于所有实验，使用市售的聚（乳酸-乙醇酸共聚物）酸膜作为对照。结果对PLCL双层膜的体外分析表明，合适的机械强度与高断裂应变相结合，有助于膜的可操作性。另外，由于其较慢的降解，PLCL双层膜与商业对照相比具有增强的稳定性，并且能够支持细胞生长和成骨分化。重要性目前的研究证实，PLCL膜具有较高的生物相容性和缓慢的降解速率，有助于延长屏障功能和骨骼再生。总之，认为该研究中开发的PLCL双层膜可用于GBR治疗。