In this work, a mono- and a bi-enzymatic analytical immobilized enzyme reactors (IMERs) were developed as prototypes for biosynthetic purposes and their performances in the in-flow synthesis of nucleoside analogues of pharmaceutical interest were evaluated. Two biocatalytic routes based on nucleoside 2'-deoxyribosyltransferase from Lactobacillus reuteri (LrNDT) and uridine phosphorylase from Clostridium perfrigens (CpUP)/purine nucleoside phosphorylase from Aeromonas hydrophila (AhPNP) were investigated in the synthesis of 2'-deoxy, 2',3'-dideoxy and arabinonucleoside derivatives. LrNDT-IMER catalyzed the synthesis of 5-fluoro-2'-deoxyuridine and 5-iodo-2'-deoxyuridine in 65-59% conversion yield, while CpUP/AhPNP-IMER provided the best results for the preparation of arabinosyladenine (60% conversion yield).

Both IMERs proved to be promising alternatives to chemical routes for the synthesis of nucleoside analogues. The developed in-flow system represents a powerful tool for the fast production on analytical scale of nucleosides for preliminary biological tests.

在这项工作中，开发了单酶和双酶分析固定化酶反应器（IMERs）作为用于生物合成目的的原型，并评估了它们在流式合成药物性核苷类似物中的性能。研究了两种基于罗伊氏乳杆菌核苷2'-脱氧核糖基转移酶（Lr NDT）和来自产气荚膜梭菌的尿苷磷酸化酶（Cp UP）/嗜水气单胞菌的嘌呤核苷磷酸化酶（Ah PNP）的生物催化路线。'，3'-二脱氧和阿拉伯核苷衍生物。rNDT-IMER以65-59％的转化率催化了5-氟-2'-脱氧尿苷和5-碘-2'-脱氧尿苷的合成，而Cp UP / Ah PNP-IMER为制备阿拉伯糖基腺嘌呤提供了最好的结果（ 60％的转化率）。

事实证明，两种IMER都是合成核苷类似物的化学途径的有前途的替代方法。先进的流入系统代表了一种强大的工具，可以快速生产初步分析的核苷分析规模。