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High hydrostatic pressure induced extraction and selective transfer of β-phosvitin from the egg yolk granule to plasma fractions.
Food Chemistry ( IF 8.8 ) Pub Date : 2020-03-26 , DOI: 10.1016/j.foodchem.2020.126696
Pauline Duffuler 1 , Mélanie Giarratano 1 , Nassim Naderi 2 , Shyam Suwal 3 , Alice Marciniak 4 , Véronique Perreault 1 , Clément Offret 5 , Guillaume Brisson 1 , James D House 2 , Yves Pouliot 1 , Alain Doyen 1
Affiliation  

Egg yolk phosvitin is of particular interest due to its functional and biological properties. Recently, it was demonstrated that high hydrostatic pressure (HHP) (400 MPa for 5 min) induced the transfer of folic acid and phosvitin from the egg yolk granule to the plasma fraction. A granule fraction (Gin) produced by egg yolk centrifugation was pressure-treated at 400 and 600 MPa for 5 and 10 min, and centrifuged to generate granule fractions (GP1 to GP4) and plasmas (PP1 to PP4). Iron and phosphorus contents were also increased in PP1 to PP4 fractions, confirming the transfer of phosvitins from pressure-treated granule to plasma. Pressurization drastically improved phosvitin recovery in PP fractions, specifically at 600 MPa for 10 min, which had the highest value of phosvitin/100 mg of dry plasma at 33.3 ± 4.39 mg. Consequently, HHP represents an alternative approach for phosvitin transfer and recovery in the egg yolk soluble fraction.

中文翻译:

高静水压力诱导β-磷酸肌醇从蛋黄颗粒中提取并选择性转移至血浆部分。

卵黄磷光蛋白由于其功能和生物学特性而特别受到关注。最近,已证明高静水压(HHP)(400 MPa,5分钟)诱导了叶酸和磷脂从蛋黄颗粒向血浆部分的转移。将通过蛋黄离心产生的颗粒级分(Gin)在400和600 MPa下加压处理5和10分钟,并离心以产生颗粒级分(GP1至GP4)和血浆(PP1至PP4)。PP1至PP4馏分中的铁和磷含量也有所增加,从而证实了磷脂素从加压处理的颗粒向血浆的转移。加压可显着提高PP馏分中的磷光蛋白回收率,特别是在600 MPa下10分钟,其磷光蛋白/ 100 mg干血浆的最高值为33.3±4.39 mg。所以,
更新日期:2020-03-27
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