An improved phosphorous determination was developed using ethanol, phosphorus determination reagent (PDR) and Ultraviolet-visible spectroscopy (UV-Vis) for analyzing the bisphosphonates (BPs). The method was carried out under mild conditions without digestion, high temperature, high pressure, and other extreme conditions. Alcohols played an important role in this method. Without alcohol, this reaction system did not have a color reaction. Alendronate (ALN) and risedronate (RIS) were used to demonstrate the reliability of the improved phosphorous determination under different reaction conditions. The absorbance had an equal ratio of increase as well as a good trend line when the content of BPs increased. The improved phosphorous determination could be a new method to measure the drug content of BPs.

中文翻译：

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基于改进的磷测定试剂的紫外可见光谱法分析双膦酸酯。

使用乙醇，磷测定试剂（PDR）和紫外可见光谱（UV-Vis）分析双膦酸盐（BPs），开发了改进的磷测定方法。该方法在温和的条件下进行，没有消化，高温，高压和其他极端条件。酒精在此方法中起着重要作用。没有酒精，该反应体系就不会发生显色反应。阿仑膦酸盐（ALN）和利塞膦酸盐（RIS）用于证明在不同反应条件下改进的磷测定的可靠性。当BP含量增加时，吸光度具有相等的增加比率，并且具有良好的趋势线。改进的磷测定方法可能是测量BPs药物含量的新方法。